凡纳滨对虾中的ELOVL6同源物在脂肪酸代谢和免疫反应中发挥双重作用。
The ELOVL6 homolog in Penaeus vannamei plays a dual role in fatty acid metabolism and immune response.
作者信息
Zhou Yuqing, Aweya Jude Juventus, Huang Zishu, Chen Ying, Tang Ziqiang, Shi Zihao, Zheng Zhihong, Zhang Yueling
机构信息
Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China.
Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; College of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen 361021 Fujian, China.
出版信息
Mol Immunol. 2023 Dec;164:7-16. doi: 10.1016/j.molimm.2023.10.008. Epub 2023 Oct 22.
In mammals, elongation of very long chain fatty acid protein 6 (ELOVL6), a key enzyme in long chain fatty acids elongation, has been reported to regulate other metabolism processes and immunity, including inflammation in vertebrates. However, little is currently known about the ELOVL6 homolog in invertebrates, especially its role in immune response. In this study, the ELOVL6 ortholog in Penaeus vannamei (designated PvELOVL6) was cloned and found to have an open reading frame (ORF) of 435 bp and encode a putative protein of 144 amino acids. Transcripts of PvELOVL6 are constitutively expressed in all shrimp tissues tested and induced in the hepatopancreas and hemocytes by Vibrio parahaemolyticus and Streptococcus iniae. Besides, PvELOVL6 knockdown followed by Vibrio parahaemolyticus challenge revealed that PvELOVL6 regulates the expression of several genes involved in fatty acid metabolism and immunity, including PvLGBP, PvLectin, PvMnSOD, PvProPO, PvFABP, PvLipase, PvCOX and PvGPDH. Moreover, transcript levels of PvELOVL6, fatty acids metabolism-related genes (i.e., PvGPDH, PvFABP, PvPERO and PvSPLA2), and immune-related genes (i.e., PvProPO, PvLectin, PvLGBP, PvLysozyme and PvCatalase) increased after silencing of the sterol regulatory element binding protein (PvSREBP). Thus, PvELOVL6 is involved in immune response and regulated by PvSREBP through an unknown mechanism in penaeid shrimp.
在哺乳动物中，超长链脂肪酸延伸蛋白6（ELOVL6）是长链脂肪酸延伸过程中的一种关键酶，据报道它能调节其他代谢过程和免疫，包括脊椎动物的炎症反应。然而，目前对于无脊椎动物中的ELOVL6同源物知之甚少，尤其是其在免疫反应中的作用。在本研究中，克隆了凡纳滨对虾中的ELOVL6直系同源物（命名为PvELOVL6），发现其开放阅读框（ORF）为435 bp，编码一个推定的144个氨基酸的蛋白质。PvELOVL6的转录本在所有检测的对虾组织中组成性表达，并在副溶血性弧菌和海豚链球菌刺激后在肝胰腺和血细胞中被诱导表达。此外，在用副溶血性弧菌攻击后进行PvELOVL6基因敲低，结果显示PvELOVL6调节几个参与脂肪酸代谢和免疫的基因的表达，包括PvLGBP、Pv凝集素、PvMnSOD、PvProPO、PvFABP、Pv脂肪酶、PvCOX和PvGPDH。此外，在固醇调节元件结合蛋白（PvSREBP）沉默后，PvELOVL6、脂肪酸代谢相关基因（即PvGPDH、PvFABP、PvPERO和PvSPLA2）以及免疫相关基因（即PvProPO、Pv凝集素、PvLGBP、Pv溶菌酶和Pv过氧化氢酶）的转录水平升高。因此，PvELOVL6参与对虾的免疫反应，并通过一种未知机制受PvSREBP调控。
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