School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Minhang District, Shanghai 200240, P. R. China.
College of Life Sciences, Shanghai Normal University, No. 100 Guilin Road, Xuhui District, Shanghai 200234, P. R. China.
J Agric Food Chem. 2023 Nov 8;71(44):16604-16617. doi: 10.1021/acs.jafc.3c04524. Epub 2023 Oct 25.
The potential biological function of tea and its active components on colitis has attracted wide attention. In this study, different tea active ingredients including tea polyphenols (TPPs), tea polysaccharides (TPSs), theabrownin (TB), and theanine (TA) have been compared in the intervention of dextran sulfate sodium (DSS)-induced colitis in mice. Specifically, TPP showed the greatest effect on colitis since it reduced 60.87% of disease activity index (DAI) compared to that of the DSS-induced colitis group, followed by the reduction of 39.13% of TPS and 28.26% of TB on DAI, whereas there was no obvious alleviative effect of TA on colitis. TPP, TPS, and TB could regulate the composition and abundance of gut microbiota to increase the content of short-chain fatty acids (SCFAs) and enhance intestinal barrier function. Further evidence was observed that TPP and TPS regulated the activation of Nrf2/ARE and the TLR4/MyD88/NF-κB P65 pathway to alleviate the colitis. Results of cell experiments demonstrated that TPP showed the greatest antiapoptosis and mitochondrial function protective capability among the tea ingredients via inhibiting the Cytc/Cleaved-caspase-3 signaling pathway. In summary, the superior anticolitis activity of TPP compared to TPS and TB is primarily attributed to its unique upregulation of the abundance of and its ability to regulate the mitochondrial function.
茶及其活性成分对结肠炎的潜在生物学功能引起了广泛关注。在本研究中，比较了不同的茶活性成分，包括茶多酚（TPPs）、茶多糖（TPSs）、茶褐素（TB）和茶氨酸（TA），干预葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎。具体来说，TPP 对结肠炎的作用最大，因为它使疾病活动指数（DAI）降低了 60.87%，而 DSS 诱导的结肠炎组降低了 39.13%的 TPS 和 28.26%的 TB，而 TA 对结肠炎没有明显的缓解作用。TPP、TPS 和 TB 可以调节肠道微生物群落的组成和丰度，增加短链脂肪酸（SCFAs）的含量，增强肠道屏障功能。进一步的证据表明，TPP 和 TPS 通过调节 Nrf2/ARE 和 TLR4/MyD88/NF-κB P65 通路的激活来缓解结肠炎。细胞实验结果表明，TPP 在茶成分中表现出最大的抗凋亡和线粒体功能保护能力，通过抑制 Cytc/Cleaved-caspase-3 信号通路。总之，与 TPS 和 TB 相比，TPP 具有优越的抗结肠炎活性，主要归因于其独特地上调丰度和调节线粒体功能的能力。
