Bitew Dagnew, Tesfaye Anteneh, Andualem Berhanu
Department of Biology, Mizan-Tepi University, P. BOX: 260, Ethiopia.
Institute of Biotechnology, University of Gondar, P.BOX: 196, Ethiopia.
Biotechnol Rep (Amst). 2023 Oct 4;40:e00815. doi: 10.1016/j.btre.2023.e00815. eCollection 2023 Dec.
The growing demand for renewable energy sources such as bioethanol is facing a lack of efficient ethanologenic microbes. This study aimed to isolate and screen ethanologenic yeasts from Ethiopian fermented beverages. A progressive screening and selection approach was employed. Selected isolates were evaluated for bioethanol production using banana peel waste as substrate. A total of 102 isolates were obtained. Sixteen isolates were selected based on their tolerance to stress conditions and carbohydrate fermentation and assimilation capacity. Most found moderately tolerant to 10 %, but slightly tolerant at 15 and 20 % (v/v) ethanol concentration. They yield 15.3 to 20.1 g/L and 9.1 ± 0.6 to 12.9 ± 1.3 g/L ethanol from 2 % (w/v) glucose and 80 g/L banana peel, respectively. Molecular characterization identified them as strains. Results demonstrate insight about their potential role in the ethanol industry. Optimization of the fermentation conditions is recommended.
对生物乙醇等可再生能源日益增长的需求正面临高效产乙醇微生物短缺的问题。本研究旨在从埃塞俄比亚发酵饮料中分离和筛选产乙醇酵母。采用了逐步筛选和选择的方法。使用香蕉皮废料作为底物，对选定的分离株进行生物乙醇生产评估。共获得102个分离株。根据它们对胁迫条件的耐受性以及碳水化合物发酵和同化能力，选择了16个分离株。大多数分离株对10%的乙醇浓度具有中等耐受性，但对15%和20%（v/v）的乙醇浓度耐受性稍低。它们分别从2%（w/v）葡萄糖和80 g/L香蕉皮中产生15.3至20.1 g/L以及9.1±0.6至12.9±1.3 g/L乙醇。分子特征鉴定将它们确定为菌株。结果显示了它们在乙醇工业中的潜在作用。建议优化发酵条件。
