Philosophy Doctor in Medicine Programme, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia.
Department of Histology, Faculty of Medicine, Universitas Islam Sumatera Utara, Medan, Indonesia.
Med Arch. 2023;77(4):254-257. doi: 10.5455/medarh.2023.77.254-257.
Hyperglycemia conditions in diabetes mellitus (DM) can turn on pro-inflammatory cytokines like IL-6 and TNF-α. These cytokines play a role in insulin resistance and the development of DM complications. People in Indonesia have used to treat diabetes, but the leaf of this plant has not been studied to see if it can reduce inflammation.
This study aims to analyze the effect of ethanolic extract of leaves (EEPML) in serum IL-6 and TNF-α levels of diabetic rats.
This study was an experiment with a post-test-only control group design. Thirty 8-week-old male Wistar rats were used in the study. They were split into six groups: K1 was the normal control group; K2 was the DM control group; K3, K4, and K5 were given EEPML at doses of 125, 250, and 500 mg/KgBW; and K6 was given metformin 45 mg/KgBW orally once a day for 14 days. A high-fat diet and a 30 mg/KgBWi.p injection of streptozotocin were used to make the diabetic rat model. ELISA method for measuring serum IL-6 and TNF-α levels. The Kruskal-Wallis and the Mann-Whitney test were used to examine the differences between the groups.
There were significant differences between treatment groups in the mean levels of serum IL-6 (p=0.017), but there were no significant differences in the mean levels of serum TNF-α (p>0.05).
Administration of leaf ethanol extract 125 mg/KgBW reduced serum IL-6 levels but could not significantly reduce serum TNF-α levels in diabetic rats.
糖尿病（DM）中的高血糖状态会激活白细胞介素 6（IL-6）和肿瘤坏死因子-α（TNF-α）等促炎细胞因子。这些细胞因子在胰岛素抵抗和 DM 并发症的发展中发挥作用。印度尼西亚人曾用 来治疗糖尿病，但尚未研究该植物的叶子是否具有抗炎作用。
本研究旨在分析 叶乙醇提取物（EEPML）对糖尿病大鼠血清中 IL-6 和 TNF-α水平的影响。
这是一项仅有后测的对照实验研究。研究共使用了 30 只 8 周龄雄性 Wistar 大鼠，将其分为 6 组：K1 为正常对照组；K2 为 DM 对照组；K3、K4 和 K5 分别给予 EEPML 剂量为 125、250 和 500 mg/kgBW；K6 给予二甲双胍 45 mg/kgBW 每天口服 1 次，共 14 天。通过高脂饮食和 30 mg/kgBW 腹腔注射链脲佐菌素建立糖尿病大鼠模型。采用 ELISA 法测定血清中 IL-6 和 TNF-α水平。采用 Kruskal-Wallis 和 Mann-Whitney 检验比较组间差异。
治疗组大鼠血清中 IL-6 水平的均值存在显著差异（p=0.017），但血清 TNF-α 水平的均值差异无统计学意义（p>0.05）。
给予 125 mg/kgBW 的 叶乙醇提取物可降低糖尿病大鼠血清中 IL-6 水平，但不能显著降低血清 TNF-α 水平。
