环状 RNA hsa-circ-0006203-hsa-circ-0004872 作为口腔癌新型检测标志物。

Circular RNA hsa-circ-0006203 - hsa-circ-0004872 as novel detecting biomarkers in oral cancer.

出版信息

Klin Onkol. 2023 Fall;36(4):378-381. doi: 10.48095/ccko2023378.

DOI:10.48095/ccko2023378

Abstract

BACKGROUND

Oral squamous cell carcinoma (OSCC) is the most common cancer of the head and neck region. The circular RNA (circRNA) is known to serve an important role in the carcinogenesis of different types of cancer. However, the circRNA role of OSCC remains unclear.

MATERIAL AND METHODS

OSCC tissues and adjacent normal tissues were obtained to detect circRNAs expression by the next generation sequencing (NGS), and OSCC tissues were selected to verify the differentially significant circRNAs by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). To further investigate the role of hsa-circ-0006203 - hsa-circ-0004872, the primer design and RT-PCR were performed. The expression levels were detected by RT-qPCR.

RESULTS

The NGS results demonstrated that circRNAs were abundantly expressed in OSCC, and two circRNAs were significantly differentially expressed. hsa-circ-0006203 - hsa-circ-0004872 were significantly downregulated in OSCC tissue samples and was statistically correlated with pathological differentiation.

CONCLUSION

In summary, the results of the present study revealed that OSCC tissues have abundant circRNAs and, to the best of our knowledge, it was our team who firstly explore the regulatory role of the hsa-circ-0006203 - hsa-circ-0004872 network in OSCC. The results indicated that hsa-circ-0006203 - hsa-circ-0004872 may be a potential biomarker for OSCC.

摘要

背景

口腔鳞状细胞癌（OSCC）是头颈部最常见的癌症。已知环状 RNA（circRNA）在不同类型癌症的发生中发挥重要作用。然而，OSCC 的 circRNA 作用仍不清楚。

材料与方法

通过下一代测序（NGS）获得 OSCC 组织和相邻正常组织以检测 circRNAs 的表达，并选择 OSCC 组织通过逆转录定量聚合酶链反应（RT-qPCR）验证差异显著的 circRNAs。为了进一步研究 hsa-circ-0006203-hsa-circ-0004872 的作用，进行了引物设计和 RT-PCR。通过 RT-qPCR 检测表达水平。

结果

NGS 结果表明 circRNAs 在 OSCC 中大量表达，有两个 circRNAs 表达显著差异。hsa-circ-0006203-hsa-circ-0004872 在 OSCC 组织样本中表达明显下调，与病理分化呈统计学相关。

结论

综上所述，本研究结果表明 OSCC 组织中存在丰富的 circRNAs，据我们所知，我们团队首次探索了 hsa-circ-0006203-hsa-circ-0004872 网络在 OSCC 中的调节作用。结果表明 hsa-circ-0006203-hsa-circ-0004872 可能是 OSCC 的潜在生物标志物。