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含有 Lhcx 家族成员和二聚体 FCPII 的硅藻光系统 II 超复合体的结构。

Structure of a diatom photosystem II supercomplex containing a member of Lhcx family and dimeric FCPII.

机构信息

Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Sci Adv. 2023 Oct 27;9(43):eadi8446. doi: 10.1126/sciadv.adi8446. Epub 2023 Oct 25.

DOI:10.1126/sciadv.adi8446
PMID:37878698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10599620/
Abstract

Diatoms rely on fucoxanthin chlorophyll -binding proteins (FCPs) for their great success in oceans, which have a great diversity in their pigment, protein compositions, and subunit organizations. We report a unique structure of photosystem II (PSII)-FCPII supercomplex from at 2.68-Å resolution by cryo-electron microscopy. FCPIIs within this PSII-FCPII supercomplex exist in dimers and monomers, and a homodimer and a heterodimer were found to bind to a PSII core. The FCPII homodimer is formed by Lhcf7 and associates with PSII through an Lhcx family antenna Lhcx6_1, whereas the heterodimer is formed by Lhcf6 and Lhcf11 and connects to the core together with an Lhcf5 monomer through Lhca2 monomer. An extended pigment network consisting of diatoxanthins, diadinoxanthins, fucoxanthins, and chlorophylls is revealed, which functions in efficient light harvesting, energy transfer, and dissipation. These results provide a structural basis for revealing the energy transfer and dissipation mechanisms and also for the structural diversity of FCP antennas in diatoms.

摘要

硅藻在海洋中取得巨大成功依赖于岩藻黄素叶绿素结合蛋白（FCPs），它们在色素、蛋白组成和亚基组织上具有很大的多样性。我们通过冷冻电镜报告了来自 的光系统 II（PSII）-FCPII 超复合体的独特结构，分辨率为 2.68 Å。该 PSII-FCPII 超复合体中的 FCPII 以二聚体和单体形式存在，发现一个同源二聚体和一个异源二聚体与 PSII 核心结合。FCPII 同源二聚体由 Lhcf7 形成，并通过 Lhcx 家族天线 Lhcx6_1 与 PSII 结合，而异源二聚体由 Lhcf6 和 Lhcf11 形成，并与核心一起通过 Lhca2 单体与 Lhcf5 单体结合。揭示了一个由硅藻叶黄素、二氢硅藻黄素、岩藻黄素和叶绿素组成的扩展色素网络，其功能在于高效的光捕获、能量转移和耗散。这些结果为揭示能量转移和耗散机制以及硅藻 FCP 天线的结构多样性提供了结构基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/f4fc406d2400/sciadv.adi8446-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/75666b2c78ad/sciadv.adi8446-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/4363dfd22a20/sciadv.adi8446-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/3e2f103a5525/sciadv.adi8446-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/7d2c466de800/sciadv.adi8446-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/b76260e7084a/sciadv.adi8446-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/f4fc406d2400/sciadv.adi8446-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/75666b2c78ad/sciadv.adi8446-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/4363dfd22a20/sciadv.adi8446-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/3e2f103a5525/sciadv.adi8446-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/7d2c466de800/sciadv.adi8446-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/b76260e7084a/sciadv.adi8446-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44db/10599620/f4fc406d2400/sciadv.adi8446-f6.jpg

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