Opuda-Asibo J, Maheswaran S K, Leininger J R
Vet Microbiol. 1986 Nov;12(4):337-51. doi: 10.1016/0378-1135(86)90083-0.
The modified enzyme-linked immunosorbent assay (ELISA) was used to determine the relative quantities of class-specific antibodies to Pasteurella haemolytica. IgG1, IgG2 and IgA were present in significantly higher quantities in bronchoalveolar washings (BAW), but in decreasing quantities, respectively; IgM was present in very low amounts. IgM, IgG1 and IgG2 were present in serum, again in decreasing quantities, respectively. IgA antibody quantities were lowest in serum. The indirect antibody ELISA was found to be superior to the indirect bacterial agglutination (IBA) technique for determining antibody titres against P. haemolytica.
采用改良的酶联免疫吸附测定（ELISA）法测定针对溶血巴斯德菌的类特异性抗体的相对量。支气管肺泡灌洗液（BAW）中IgG1、IgG2和IgA的含量显著更高，但分别呈递减趋势；IgM的含量极低。血清中存在IgM、IgG1和IgG2，同样分别呈递减趋势。血清中IgA抗体量最低。发现间接抗体ELISA在测定针对溶血巴斯德菌的抗体效价方面优于间接细菌凝集（IBA）技术。
