Mosier D A, Simons K R, Confer A W, Panciera R J, Clinkenbeard K D
Department of Veterinary Pathology, Oklahoma State University, Stillwater 74078.
Vet Immunol Immunopathol. 1989 Aug;22(1):53-65. doi: 10.1016/0165-2427(89)90163-3.
The serum IgG and IgM antibody responses of 48 cattle vaccinated with live Pasteurella haemolytica (LIVE), formalin-killed P. haemolytica in Freund's incomplete adjuvant (FIA), or formalin-killed P. haemolytica in aluminum hydroxide adjuvant (ALH) to a variety of P. haemolytica antigens were evaluated. Enzyme-linked immunosorbent assays (ELISAs) were used to determine the sequential and day 21 IgG and IgM antibody responses to whole P. haemolytica (WB), a capsular carbohydrate-protein subunit (CPS) extracted from the organism, P. haemolytica capsular carbohydrate (CC), and P. haemolytica leukotoxin (LT). LIVE and FIA vaccinates developed generally higher IgG and IgM responses to all antigens compared to ALH vaccinates. LIVE vaccinates developed IgG responses to LT which were significantly higher (P less than 0.05) than all other vaccinates. In contrast, FIA vaccinates developed significantly higher IgG responses to CPS than all other vaccinates. On the basis of the ELISA results, similar or cross reacting antigenic sites were present in preparations containing surface antigens (WB, CPS and CC), but not LT. Disease resistance, as determined by experimental lesions induced in the 48 calves by transthoracic challenge with P. haemolytica, was significantly greater in the LIVE and FIA vaccinates compared with ALH vaccinates. No significant difference in resistance was detected between LIVE and FIA vaccinates. Lesions in ALH vaccinates were not significantly different than those in phosphate-buffered saline (PBS) controls. Increased IgG responses to all antigens were significantly associated with resistance to experimental disease; however, IgG responses to CPS were most highly correlated with resistance. The only IgM response which was significantly correlated with resistance was the response to CPS. These studies indicate that serum IgG antibody responses to various surface antigens of P. haemolytica, as well as LT, can enhance resistance to experimental pneumonic pasteurellosis. Serum IgM responses, however, do not appear to play a major role in resistance to experimental disease.
评估了48头接种了活溶血巴斯德氏菌（LIVE）、弗氏不完全佐剂中福尔马林灭活的溶血巴斯德氏菌（FIA）或氢氧化铝佐剂中福尔马林灭活的溶血巴斯德氏菌（ALH）的牛对多种溶血巴斯德氏菌抗原的血清IgG和IgM抗体反应。采用酶联免疫吸附测定（ELISA）来确定对全溶血巴斯德氏菌（WB）、从该菌提取的荚膜碳水化合物 - 蛋白质亚基（CPS）、溶血巴斯德氏菌荚膜碳水化合物（CC）和溶血巴斯德氏菌白细胞毒素（LT）的连续及第21天的IgG和IgM抗体反应。与ALH疫苗接种牛相比，LIVE和FIA疫苗接种牛对所有抗原通常产生更高的IgG和IgM反应。LIVE疫苗接种牛对LT产生的IgG反应显著高于所有其他疫苗接种牛（P小于0.05）。相反，FIA疫苗接种牛对CPS产生的IgG反应显著高于所有其他疫苗接种牛。基于ELISA结果，含有表面抗原（WB、CPS和CC）的制剂中存在相似或交叉反应的抗原位点，但LT中不存在。通过用溶血巴斯德氏菌经胸攻击在48头犊牛中诱导的实验性病变确定的抗病性，LIVE和FIA疫苗接种牛比ALH疫苗接种牛显著更强。LIVE和FIA疫苗接种牛之间未检测到抗性的显著差异。ALH疫苗接种牛的病变与磷酸盐缓冲盐水（PBS）对照的病变无显著差异。对所有抗原的IgG反应增加与对实验性疾病的抗性显著相关；然而，对CPS的IgG反应与抗性相关性最高。唯一与抗性显著相关的IgM反应是对CPS的反应。这些研究表明，对溶血巴斯德氏菌各种表面抗原以及LT的血清IgG抗体反应可增强对实验性肺炎巴氏杆菌病的抗性。然而，血清IgM反应似乎在对实验性疾病的抗性中不发挥主要作用。
