Masalski N, Ivanov I N, Dikova Ts, Marinova A, Gogov I
Vet Med Nauki. 1986;23(7):28-32.
Staphylococci were isolated from the udder of dead sheep and sheep that were emergency-slaughtered because of a gangrenous mastitis infection. The organisms proved to be hemolytic and mannite- and catalase-positive, and were referred to various phage types, being most frequently lysed by phages 78 and 42E. Toxin production depended on the quality of the medium used, the amount of atmospheric carbon dioxide, the period of incubation, and the individual strain qualities. Strains of high hemolysin production were used to prepare a vaccine which was applied to sheep both experimentally and in the conditions of the practice at the time of active lactation. Following treatment of the vaccinated animals parallel to a number of controls with one and the same challenging agent morbidity with the first group ran as high as 25 per cent with no death cases, while with the second group it was 100 per cent, and mortality with the latter was 50 per cent. Immunity with the use of the vaccine lasted but four months, therefore, revaccination was needed by the end of this period.
葡萄球菌是从因坏疽性乳腺炎感染而死亡或被紧急屠宰的绵羊乳房中分离出来的。这些微生物被证明具有溶血活性，且甘露醇和过氧化氢酶呈阳性，并被归为不同的噬菌体类型，其中最常被噬菌体78和42E裂解。毒素的产生取决于所用培养基的质量、大气中二氧化碳的含量、培养时间以及各个菌株的特性。使用高溶血素产生菌株制备了一种疫苗，该疫苗在实验条件下以及在实际泌乳期应用于绵羊。用同一攻击因子对接种疫苗的动物和若干对照组进行平行处理后，第一组的发病率高达25%，无死亡病例，而第二组的发病率为100%，死亡率为50%。使用该疫苗产生的免疫力仅持续四个月，因此，在此期间结束时需要再次接种疫苗。
