Kaludin I, Dimitrova I
Vet Med Nauki. 1986;23(7):41-6.
The zinc content in spermatozoa and the tocopherol content in the total semen of rams were determined following the incubation of semen in the course of one hour at 37 degrees C, adding no selenium and methionine. Zinc was evaluated by means of AAS1, and tocopherol was measured spectrophotometrically. It was found that both selenium ions and methionine produced a varying effect on the zinc transport and the tocopherol content. The loss of zinc in spermatozoa increased with the rise of selenium level up to certain values, after which the effect of selenium dropped. Zinc was lost also under the effect of methionine, however, higher levels of methionine did not lead to the further loss of zinc. The incubated samples of semen with the addition of selenium and methionine contained higher amounts of tocopherol as against the control samples.
在37摄氏度下将公羊精液孵育一小时，且不添加硒和蛋氨酸，之后测定精子中的锌含量以及精液总体中的生育酚含量。锌通过原子吸收光谱法（AAS1）进行评估，生育酚则采用分光光度法进行测定。结果发现，硒离子和蛋氨酸对锌的转运及生育酚含量均产生了不同影响。精子中锌的损失随着硒水平的升高而增加，直至达到一定值，之后硒的影响减弱。在蛋氨酸的作用下锌也会流失，然而，较高水平的蛋氨酸并不会导致锌的进一步流失。添加了硒和蛋氨酸的精液孵育样本中，生育酚的含量高于对照样本。
