College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
College of Agriculture and Forestry Sciences, Linyi University, Linyi, Shandong, 276000, China.
BMC Plant Biol. 2023 Oct 25;23(1):513. doi: 10.1186/s12870-023-04474-7.
Resistance genes (R genes) are a class of genes that are immune to a wide range of diseases and pests. In planta, NLR genes are essential components of the innate immune system. Currently, genes belonging to NLR family have been found in a number of plant species, but little is known in peach. Here, 286 NLR genes were identified on peach genome by using their homologous genes in Arabidopsis thaliana as queries. These 286 NLR genes contained at least one NBS domain and LRR domain. Phylogenetic and N-terminal domain analysis showed that these NLRs could be separated into four subfamilies (I-IV) and their promoters contained many cis-elements in response to defense and phytohormones. In addition, transcriptome analysis showed that 22 NLR genes were up-regulated after infected by Green Peach Aphid (GPA), and showed different expression patterns. This study clarified the NLR gene family and their potential functions in aphid resistance process. The candidate NLR genes might be useful in illustrating the mechanism of aphid resistance in peach.
抗性基因（R 基因）是一类能够抵抗多种疾病和害虫的基因。在植物体内，NLR 基因是先天免疫系统的重要组成部分。目前，已经在许多植物物种中发现了属于 NLR 家族的基因，但在桃中知之甚少。在这里，通过使用拟南芥中的同源基因作为查询，在桃基因组中鉴定出了 286 个 NLR 基因。这些 286 个 NLR 基因至少包含一个 NBS 结构域和 LRR 结构域。系统发育和 N 端结构域分析表明，这些 NLR 可以分为四个亚家族（I-IV），它们的启动子包含许多响应防御和植物激素的顺式元件。此外，转录组分析表明，22 个 NLR 基因在被绿桃蚜（GPA）感染后上调，表现出不同的表达模式。这项研究阐明了 NLR 基因家族及其在蚜虫抗性过程中的潜在功能。候选 NLR 基因可能有助于阐明桃蚜抗性的机制。
