Babraham Institute, Babraham Research Campus, Cambridge, UK.
Rapid Commun Mass Spectrom. 2023 Feb;38 Suppl 1:e9641. doi: 10.1002/rcm.9641. Epub 2023 Oct 26.
Extraction protocols and liquid chromatography coupled with mass spectrometry (LC-MS) and tandem mass spectrometry (LC-MS/MS) methods for the measurement of four lipid categories, namely glycerophospholipids, glycerolipids, sphingolipids and sterol lipids in human plasma, are described here. Step-by-step instructions are provided for the liquid-liquid extraction methods, including solution preparation and the non-endogenous lipid internal standards used. All instrumental conditions, chromatography columns and solutions required for the LC-MS and LC-MS/MS methods are also provided in detail.
本文描述了用于测量人血浆中四类脂质（即甘油磷脂、甘油酯、鞘脂和固醇脂质）的提取方案以及液相色谱-质谱（LC-MS）和串联质谱（LC-MS/MS）方法。本文提供了液-液萃取方法的分步说明，包括溶液制备和所用非内源性脂质内标。还详细提供了 LC-MS 和 LC-MS/MS 方法所需的所有仪器条件、色谱柱和溶液。
