抗氧化剂原花青素B2（PCB2）对绵羊卵母细胞体外成熟（IVM）及玻璃化冷冻应激下发育潜能的影响
Effect of antioxidant procyanidin b2 (pcb2) on ovine oocyte developmental potential in response to in vitro maturation (ivm) and vitrification stress.
作者信息
Bai J, Li J, Wang L, Hao S, Guo Y, Liu Y, Zhang Z, Li H, Sun W Q, Shi G, Wan P, Fu X
机构信息
Institute of Biothermal Science and Technology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China.
Department of Reproductive Medicine, Reproductive Medical Center, The First Hospital of Hebei Medical University, Shijiazhuang, China.
出版信息
Cryo Letters. 2023 Mar-Apr;44(2):109-117.
BACKGROUND
It was demonstrated that external stress, such as in vitro maturation (IVM) and vitrification process can induce significantly reduced development capacity in oocytes. Previous studies indicated that antioxidants play a pivotal part in the acquisition of adaptation in changed conditions. At present, the role of the natural potent antioxidant PCB2 in response to IVM and vitrification during ovine oocyte manipulation has not been explored.
OBJECTIVE
To investigate whether PCB2 treatment could improve the developmental potential of ovine oocytes under IVM and vitrification stimuli.
MATERIALS AND METHODS
The experiment was divided into two parts. Firstly, the effect of PCB2 on the development of oocytes during IVM was evaluated. Un-supplemented and 5 ug per mL PCB2-supplemented in the IVM solution were considered as control and experimental groups (C + 5 ug per mL PCB2). The polar body extrusion (PBE) rate, mitochondrial membrane potential (MMP), ATP, reactive oxygen species (ROS) levels and early apoptosis of oocytes were measured after IVM. Secondly, we further determine whether PCB2 could improve oocyte quality under vitrification stress. The survival rate, PBE rate and early apoptosis of oocytes were compared between fresh group, vitrified group and 5 ug per mL PCB2-supplemented in the IVM solution after vitrification (V + 5 ug per mL PCB2).
RESULTS
Compared to the control group, adding PCB2 significantly increased PBE rate (79.4% vs. 62.8%, P < 0.01) and MMP level (1.9 +/- 0.08 vs. 1.3 +/- 0.04, P < 0.01), and decreased ROS level (47.1 +/- 6.3 vs. 145.3 +/- 8.9, P < 0.01). However, there was no significant difference in ATP content and early apoptosis. Compared to the fresh group, vitrification significantly reduced oocytes viability (43.0% vs. 90.8%, P < 0.01) as well as PBE rate (24.2% vs. 60.6%, P < 0.05). However, 5 ug per mL PCB2-supplemention during maturation had no effect on survival, PBE or early apoptosis in vitrified oocytes.
CONCLUSION
PCB2 could effectively antagonise the oxidative stress during IVM and promote oocyte development. DOI: 10.54680/fr23210110412.
背景
已证实外部应激，如体外成熟（IVM）和玻璃化过程可显著降低卵母细胞的发育能力。先前的研究表明，抗氧化剂在适应变化条件的过程中起关键作用。目前，尚未探究天然强效抗氧化剂PCB2在绵羊卵母细胞操作过程中对IVM和玻璃化的反应作用。
目的
研究PCB2处理是否能提高IVM和玻璃化刺激下绵羊卵母细胞的发育潜能。
材料与方法
实验分为两部分。首先，评估PCB2对IVM期间卵母细胞发育的影响。将IVM溶液中未添加和添加5μg/mL PCB2的情况分别视为对照组和实验组（C + 5μg/mL PCB2）。IVM后测量卵母细胞的极体排出（PBE）率、线粒体膜电位（MMP）、ATP、活性氧（ROS）水平和早期凋亡情况。其次，进一步确定PCB2是否能在玻璃化应激下提高卵母细胞质量。比较新鲜组、玻璃化组和玻璃化后IVM溶液中添加5μg/mL PCB2组（V + 5μg/mL PCB2）的卵母细胞存活率、PBE率和早期凋亡情况。
结果
与对照组相比，添加PCB2显著提高了PBE率（79.4%对62.8%，P < 0.01）和MMP水平（1.9±0.08对1.3±0.04，P < 0.01），并降低了ROS水平（47.1±6.3对145.3±8.9，P < 0.01）。然而，ATP含量和早期凋亡无显著差异。与新鲜组相比，玻璃化显著降低了卵母细胞活力（43.0%对90.8%，P < 0.01）以及PBE率（24.2%对60.6%，P < 0.05）。然而，成熟过程中添加5μg/mL PCB2对玻璃化卵母细胞的存活、PBE或早期凋亡无影响。
结论
PCB2可有效对抗IVM期间的氧化应激并促进卵母细胞发育。DOI: 10.54680/fr23210110412。
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