Altboum I, Pick E
Int Arch Allergy Appl Immunol. 1979;60(1):29-43. doi: 10.1159/000232320.
Spleen cells of C57B1/6J mice immunized with complete Freund's adjuvant produced macrophage migration inhibitory factor (MIF) when incubated in vitro with tuberculin purified protein derivative (PPD). For optimal MIF production spleen cells were cultured for 48 h in a serum-free medium, at a concentration of 2 x 10(7) cells/ml. MIF was assayed in a xenogenic system, using oil-induced guinea pig peritoneal exudate cells as targets. MIF synthesis could also be induced by pulsing spleen cells for 2 h with concanavalin A, phytohemagglutinin, pokeweed mitogen or lipopolysaccharide, followed by culture in plain medium. No MIF secretion was induced by incubation of spleen cells with anti-theta or rabbit anti-mouse IgG sera. Cells producing MIF in response to PPD were characterized as B cells by virtue of being insensitive to anti-theta serum and complement, by being retained on nylon wool, glass bead and anti-Ig colums and by the presence of Fc receptors. PPD-stimulated T cells did not produce MIF. PPD-induced mouse spleen cell MIF demonstrated a moderate loss of activity by heating at 56 and 80 degrees C and was completely inactivated after digestion with chymotrypsin. By fractionation on Sephadex G-200, migration inhibitory activity was recovered in a molecular range of 100,000-12,400 daltons.
用完全弗氏佐剂免疫的C57B1/6J小鼠的脾细胞，在体外与结核菌素纯蛋白衍生物（PPD）一起孵育时会产生巨噬细胞移动抑制因子（MIF）。为了实现最佳的MIF产生，脾细胞在无血清培养基中以2×10⁷个细胞/毫升的浓度培养48小时。MIF在一个异种系统中进行测定，使用油诱导的豚鼠腹腔渗出细胞作为靶细胞。通过用刀豆球蛋白A、植物血凝素、商陆有丝分裂原或脂多糖刺激脾细胞2小时，然后在普通培养基中培养，也可以诱导MIF合成。用抗θ或兔抗小鼠IgG血清孵育脾细胞不会诱导MIF分泌。对PPD产生反应而产生MIF的细胞，由于对抗θ血清和补体不敏感、能被保留在尼龙棉、玻璃珠和抗Ig柱上以及存在Fc受体，而被鉴定为B细胞。PPD刺激的T细胞不产生MIF。PPD诱导的小鼠脾细胞MIF在56℃和80℃加热时活性有一定程度的丧失，用胰凝乳蛋白酶消化后完全失活。通过在Sephadex G -
