猫淋巴细胞群体的特性与有丝分裂

Characterization and mitogenesis of feline lymphocyte populations.

作者信息

Rojko J L, Hoover E A, Finn B L, Olsen R G

出版信息

Int Arch Allergy Appl Immunol. 1982;68(3):226-32. doi: 10.1159/000233103.

Abstract

Feline lymphocyte populations from blood, spleen, lymph node, thymus and bone marrow were examined for the following markers: rosette formation with guinea pig erythrocytes (GPE-T cells); surface feline thymocyte antigen (T cells); surface immunoglobulin (B cells); cytoplasmic immunoglobulin (preB cells, plasma cells); receptors for the Fc portion of IgG (FcyR-positive T and B cells), and the third component of complement (CR-positive cells - primarily B cells). The blastogenic responses of feline lymphocytes from peripheral blood, spleen, lymph node (LN), thymus and marrow were investigated using the following mitogens: Escherichia coli lipopolysaccharide (LPS), dextran sulfate (DxS), concanavalin A (conA), phytohemagglutinin (PHA) and pokeweed mitogen (PWM). Feline T lymphocytes identified by rosette formation with GPE and surface thymocyte antigen were present in thymus, spleen, LN, blood and, rarely, in marrow. T cell subsets, but not B cell subsets, were differentiated according to mitogen activation. Cells responding to PHA were immature, nonrecirculating cells, which were most strongly activated in thymus [stimulation index (SI) = 12], lymph node (SI = 11) and spleen (SI = 6). PWM-responsive cells were relatively mature, recirculating lymphocytes of widespread distribution and blastogenesis was greater in spleen (SI = 36) and LN (SI = 29), followed by thymus (SI = 23) and blood (SI = 15). Con A was a potent mitogen for cells of spleen (SI = 113), blood (SI = 80) and LN (SI = 77), but not thymus (SI = 7), suggesting that the con-A-inducible cell was a mature, recirculating, postthymic cell. Optimal mitogenic response to the B cell mitogen, LPS, was dependent upon increased cell concentrations (3 x 10(5) versus 1 x 10(5) per microtiter well) and increased incubation time (5 days versus 3 days). Surface IgG- and IgM-bearing lymphocytes and CR-bearing cells from spleen and blood were stimulated preferentially. DxS was moderately mitogenic for CR-bearing lymphocytes in spleen (SI = 4), LN (SI = 3) and blood (SI = 3), but not marrow (SI = 1).

摘要

对来自猫的血液、脾脏、淋巴结、胸腺和骨髓的淋巴细胞群体进行了以下标志物检测：与豚鼠红细胞形成玫瑰花结（GPE-T细胞）；表面猫胸腺细胞抗原（T细胞）；表面免疫球蛋白（B细胞）；细胞质免疫球蛋白（前B细胞、浆细胞）；IgG的Fc部分受体（FcyR阳性T和B细胞），以及补体第三成分（CR阳性细胞 - 主要是B细胞）。使用以下促有丝分裂原研究了来自外周血、脾脏、淋巴结（LN）、胸腺和骨髓的猫淋巴细胞的增殖反应：大肠杆菌脂多糖（LPS）、硫酸葡聚糖（DxS）、刀豆球蛋白A（conA）、植物血凝素（PHA）和商陆有丝分裂原（PWM）。通过与GPE形成玫瑰花结和表面胸腺细胞抗原鉴定的猫T淋巴细胞存在于胸腺、脾脏、LN、血液中，骨髓中则很少见。根据促有丝分裂原激活情况区分T细胞亚群，但不区分B细胞亚群。对PHA有反应的细胞是未成熟的、非再循环细胞，在胸腺中激活最强[刺激指数（SI）= 12]，淋巴结（SI = 11）和脾脏（SI = 6）。对PWM有反应的细胞是相对成熟的、再循环淋巴细胞，分布广泛，在脾脏中增殖（SI = 36）和LN中增殖更大（SI = 29），其次是胸腺（SI = 23）和血液（SI = 15）。Con A是脾脏（SI = 113）、血液（SI = 80）和LN（SI = 77）细胞的有效促有丝分裂原，但对胸腺细胞无效（SI = 7），这表明con-A诱导的细胞是成熟的、再循环的、胸腺后细胞。对B细胞促有丝分裂原LPS的最佳促有丝分裂反应取决于细胞浓度增加（每微孔板3×10⁵ 个细胞对1×10⁵ 个细胞）和孵育时间延长（5天对3天）。脾脏和血液中带有表面IgG和IgM的淋巴细胞以及带有CR的细胞受到优先刺激。DxS对脾脏（SI = 4）、LN（SI = 3）和血液（SI = 3）中带有CR的淋巴细胞有中度促有丝分裂作用，但对骨髓细胞无效（SI = 1）。