State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 Park 1, Beichen West Road, Chaoyang District, Beijing 100101, China.
School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China.
J Agric Food Chem. 2023 Nov 29;71(47):18385-18394. doi: 10.1021/acs.jafc.3c05783. Epub 2023 Oct 27.
(previously known as ) is a pathogenic fungus of mushrooms. The molecular mechanism underlying the infection of in fruiting bodies remains unknown. In this study, three known peptaibols, alamethicin F-50, polysporin B, and septocylindrin B (-), and a new analogue, longisporin A (), were detected and identified in the culture of and the fruiting bodies of infected by . The primary amino sequence of longisporin A is defined as Ac-Aib-Pro-Aib-Ala-Aib-Aib-Gln-Aib-Val-Aib-Glu-Leu-Aib-Pro-Val-Aib-Aib-Gln-Gln-Phaol. The peptaibols - greatly suppressed the mycelial growth of . In addition, treatment with alamethicin F-50 produced damage on the cell wall and membrane of . Compounds - also exhibited inhibitory activities against human pathogens including , , methicillin-resistant and vancomycin-resistant , and plant pathogen . Herein, peptaibols are confirmed as virulence factors involved in the invasion of on , providing insights into the interaction between pathogenic and mushrooms.
（以前称为）是一种致病真菌，存在于 蘑菇中。其在子实体中感染的分子机制尚不清楚。在这项研究中，在 培养物和 感染的 子实体中检测并鉴定出三种已知的肽类抗生素，即alamethicin F-50、polysporin B 和 septocylindrin B (-)，以及一种新的类似物 longisporin A ()。longisporin A 的一级氨基酸序列定义为 Ac-Aib-Pro-Aib-Ala-Aib-Aib-Gln-Aib-Val-Aib-Glu-Leu-Aib-Pro-Val-Aib-Aib-Gln-Gln-Phaol。肽类抗生素 - 极大地抑制了 的菌丝生长。此外，alamethicin F-50 的处理对 的细胞壁和膜造成了损伤。化合物 - 也对包括 、 、耐甲氧西林 和耐万古霉素 以及植物病原体 在内的人类病原体表现出抑制活性。在此，肽类抗生素被确认为参与 入侵 的毒力因子，为了解致病 与蘑菇之间的相互作用提供了线索。
