长枝木霉海洋来源菌株产生的长链肽菌素的鉴定及生物活性

Identification and Biological Activities of Long-Chain Peptaibols Produced by a Marine-Derived Strain of Trichoderma longibrachiatum.

作者信息

Mohamed-Benkada Mustapha, François Pouchus Yves, Vérité Philippe, Pagniez Fabrice, Caroff Nathalie, Ruiz Nicolas

机构信息

Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université des Sciences et de la Technologie d'Oran-Mohamed Boudiaf (USTO-MB), El Mnaouar, B.P. 1505, Bir El Djir, 31000, Oran, Algeria.

Faculté de Pharmacie, MMS, Université de Nantes, FR-44000, Nantes.

出版信息

Chem Biodivers. 2016 May;13(5):521-30. doi: 10.1002/cbdv.201500159.

DOI:10.1002/cbdv.201500159

PMID:27009018

Abstract

Six long-chain peptaibols, 1 - 6, were identified from agar cultures of a marine-derived Trichoderma longibrachiatum Rifai strain (MMS151) isolated from blue mussels. The structure elucidation was carried out using electrospray ionization ion trap mass spectrometry (ESI-IT-MS) and GC/EI-MS. The long-chain peptaibols exhibited the general building scheme Ac-Aib-Ala-Aib-Ala-Aib-XXX-Gln-Aib-Vxx-Aib-Gly-XXX-Aib-Pro-Vxx-Aib-XXX-Gln-Gln-Pheol and were similar or identical to recurrent 20-residue peptaibols produced by Trichoderma spp. Three new sequences were identified and were called longibrachins A-0, A-II-a, and A-IV-b. The isolated peptaibols were assayed for cytotoxic, antibacterial, and antifungal activities, and acute toxicity on Dipteran larvae.

摘要

从采自蓝贻贝的海洋来源长枝木霉里氏木霉菌株（MMS151）的琼脂培养物中鉴定出6种长链肽抗生素，即1-6。使用电喷雾电离离子阱质谱法（ESI-IT-MS）和气相色谱/电子轰击质谱法（GC/EI-MS）进行结构解析。长链肽抗生素呈现出一般的结构模式：Ac-Aib-Ala-Aib-Ala-Aib-XXX-Gln-Aib-Vxx-Aib-Gly-XXX-Aib-Pro-Vxx-Aib-XXX-Gln-Gln-Pheol，并且与木霉属产生的常见20个残基的肽抗生素相似或相同。鉴定出三个新序列，分别称为长枝霉素A-0、A-II-a和A-IV-b。对分离得到的肽抗生素进行细胞毒性、抗菌和抗真菌活性以及对双翅目幼虫的急性毒性测定。