淋巴管内皮细胞对稳定直流电电场的淋巴管生成反应。

Laboratory of Genetic Disease and Perinatal Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children of the Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.

Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, China.

Cell Adh Migr. 2023 Dec;17(1):1-14. doi: 10.1080/19336918.2023.2271260. Epub 2023 Oct 27.

Lymphangiogenesis plays pivotal roles in diverse physiological and pathological conditions. Steady direct-current electric fields (DC EFs) induce vascular endothelial behaviors related to angiogenesis have been observed. This study investigated the effects of DC EFs on the lymphangiogenic response of lymphatic endothelial cells (LECs). We demonstrated that EFs stimulation induced directional migration, reorientation, and elongation of human LECs in culture. These lymphangiogenic responses required VEGF receptor 3 (VEGFR-3) activation and were mediated through the PI3K-Akt, Erk1/2, and p38 MAPK signaling pathways in relation to the reorganization of the actin cytoskeleton. Our results indicate that endogenous EFs may play a role in lymphangiogenesis in vivo, and VEGFR-3 signaling activation may be involved in the cellular function of LECs driven by EFs.

淋巴管生成在多种生理和病理条件中起着关键作用。已经观察到稳定的直流电场 (DC EF) 诱导与血管生成相关的血管内皮行为。本研究探讨了 DC EF 对淋巴管内皮细胞 (LEC) 的淋巴管生成反应的影响。我们证明 EF 刺激诱导培养中的人 LEC 定向迁移、重定向和伸长。这些淋巴管生成反应需要 VEGF 受体 3 (VEGFR-3) 的激活，并通过与肌动蛋白细胞骨架重排相关的 PI3K-Akt、Erk1/2 和 p38 MAPK 信号通路介导。我们的结果表明，内源性 EF 可能在体内淋巴管生成中发挥作用，并且 VEGFR-3 信号激活可能参与 EF 驱动的 LEC 细胞的功能。