Yıldız Ayşegül, Birer Mehmet, Turgut Birer Yağmur, Uyar Recep, Yurdakök-Dikmen Begüm, Acartürk Füsun
Department of Pharmaceutical Technology, Faculty of Pharmacy, Gazi University, Ankara, Turkey.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
J Biomater Appl. 2023 Nov;38(5):646-661. doi: 10.1177/08853282231207578. Epub 2023 Oct 27.
Tissue engineering has recently attracted attention as an alternative to traditional treatment methods for tissue and organ damage. Since bone is one of the most important vital parts of the body, the treatment of bone damage is important. Silk fibroin is a natural polymer with properties such as biocompatibility and biodegradability, which attracts attention with its controlled release, especially in drug delivery systems. In this study, gelatin-based scaffolds loaded with silk fibroin nanoparticles and β -tricalcium phosphate (β -TCP) were developed to be used as a potential drug delivery system in bone tissue engineering. The chosen nanoparticle formulation has a 294 nm average diameter with a 0.380 polidispersity index (PDI). In vitro characterization of scaffolds was performed by mechanical, morphological characterization, swelling capacity, Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared Spectroscopy (FT-IR) measurements, and biocompatibility was evaluated by cell culture studies. Swelling index, tensile strength, elongation at break, and Young modulus of the β -TCP and silk nanoparticles loaded scaffold were found as 456%, 1.476 MPa, 6.75%, and 24 MPa, respectively. In vitro cell culture studies have shown that scaffolds prepared in the present study can accelerate osteoblast differentiation and increase the healing rate of bone tissues. In addition, they have the potential to be used as a drug delivery system in bone tissue engineering that needs to be evaluated with further studies.
作为组织和器官损伤传统治疗方法的替代方案，组织工程学近来备受关注。由于骨骼是人体最重要的 vital 部位之一，骨骼损伤的治疗至关重要。丝素蛋白是一种具有生物相容性和生物可降解性等特性的天然聚合物，其在控释方面备受关注，尤其是在药物递送系统中。在本研究中，开发了负载丝素蛋白纳米颗粒和β - 磷酸三钙（β - TCP）的明胶基支架，以用作骨组织工程中潜在的药物递送系统。所选的纳米颗粒制剂平均直径为294 nm，多分散指数（PDI）为0.380。通过力学、形态表征、溶胀能力、差示扫描量热法（DSC）、傅里叶变换红外光谱（FT - IR）测量对支架进行体外表征，并通过细胞培养研究评估生物相容性。负载β - TCP和丝纳米颗粒的支架的溶胀指数、拉伸强度、断裂伸长率和杨氏模量分别为456%、1.476 MPa、6.75%和24 MPa。体外细胞培养研究表明，本研究制备的支架可加速成骨细胞分化并提高骨组织的愈合率。此外，它们有潜力用作骨组织工程中的药物递送系统，有待进一步研究评估。
