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用于评估放射性神经学结果的严格行为测试平台。

A rigorous behavioral testing platform for the assessment of radiation-induced neurological outcomes.

机构信息

Department of Radiation Oncology, University of California at Irvine, Irvine, CA, United States.

Department of Radiation Oncology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.

出版信息

Methods Cell Biol. 2023;180:177-197. doi: 10.1016/bs.mcb.2023.02.015. Epub 2023 Mar 25.

DOI:10.1016/bs.mcb.2023.02.015
PMID:37890929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11093273/
Abstract

Behavioral testing is a popular and reliable method of neurocognitive assessment of rodents but the lack of standard operating procedures has led to a high variation of protocols in use. Therefore, there exists a strong need to standardize protocols for a combined behavioral platform in order to maintain consistency across institutions and assist newcomers in the field. This paper provides details on the methodology of several behavioral tasks which have been validated in identifying radiation induced cognitive impairment as well as provide guidance on timescales and best practices. The cognitive assessments outlined here are optimized for rodent studies and either target learning and memory (open field task, object in updated location, novel object recognition, object in place, and temporal order) or mood and cognition (social interaction, elevated plus maze, light dark box, forced swim test, and fear extinction). We have utilized this platform successfully in evaluating cognitive injury induced by various radiation types, doses, fractionation schedules and also with ultra-high dose rate FLASH radiotherapy. Recommended materials and software are provided as well as advice on methods of data analysis. In this way a comprehensive behavioral platform is described with broad applicability to assess cognitive endpoints critical to therapeutic outcome.

摘要

行为测试是一种评估啮齿动物神经认知的常用且可靠的方法，但由于缺乏标准操作程序，导致目前使用的方案存在很大差异。因此，非常有必要为联合行为平台制定标准化方案，以保持机构间的一致性，并为该领域的新手提供帮助。本文详细介绍了几种行为任务的方法学，这些任务已被验证可用于识别放射性诱导的认知障碍，并提供了有关时间尺度和最佳实践的指导。这里概述的认知评估经过优化，适用于啮齿动物研究，要么针对学习和记忆（旷场任务、更新位置的物体、新物体识别、位置物体和时间顺序），要么针对情绪和认知（社会互动、高架十字迷宫、明暗箱、强迫游泳测试和恐惧消退）。我们已经成功地利用该平台评估了各种类型的辐射、剂量、分割方案以及超高剂量率 FLASH 放射治疗引起的认知损伤。还提供了推荐的材料和软件以及数据分析方法的建议。通过这种方式，描述了一个具有广泛适用性的综合行为平台，可用于评估对治疗结果至关重要的认知终点。

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