Department of Botany, Lahore College for Women University, Lahore, Pakistan.
Institute of Botany, University of the Punjab, Lahore, Pakistan.
BMC Plant Biol. 2023 Oct 27;23(1):522. doi: 10.1186/s12870-023-04527-x.
Salinity adversely affects okra [Abelmoschus esculentus (L.) Moench] plants by inducing osmotic and oxidative stresses. This study was designed to enhance salinity-induced osmotic and oxidative stress tolerance in okra plants by applying organic amendments.
The effects of different organic amendments (municipal solid waste compost, farmyard manure (FYM) and press mud) on osmotic potential, water use efficiency, activities of antioxidant enzymes, total soluble sugar, total soluble proline, total soluble protein and malondialdehyde (MDA) contents of okra plants grown under saline conditions (50 mM sodium chloride) were evaluated in a pot experiment. The organic amendments were applied each at the rate of 5% and 10% per pot or in various combinations (compost + FYM, FYM + press mud and compost + press mud each at the rate of 2.5% and 5% per pot).
As compared to control, high total soluble sugar (60.41), total soluble proline (33.88%) and MDA (51%) contents and increased activities of antioxidant enzymes [superoxide dismutase (83.54%), catalase (78.61%), peroxidase (53.57%] in salinity-stressed okra plants, were indicative of oxidative stress. Salinity significantly reduced the osmotic potential (41.78%) and water use efficiency (4.75%) of okra plants compared to control. Under saline conditions, 5% (farmyard manure + press mud) was the most effective treatment, which significantly improved osmotic potential (27.05%), total soluble sugar (4.20%), total soluble protein (73.62%) and total soluble proline (23.20%) contents and superoxide dismutase activity (32.41%), compared to saline soil. Application of 2.5% (FYM + press mud), 5% press mud, and 10% compost significantly reduced MDA content (27%) and improved activities of catalase (38.64%) and peroxidase (48.29%), respectively, compared to saline soil, thus facilitated to alleviate oxidative stress in okra plants.
Using organic amendments (municipal solid waste compost, farmyard manure and press mud) was a cost-effective approach to improve salinity-induced osmotic and oxidative stress tolerance in okra plants.
盐分通过诱导渗透胁迫和氧化胁迫对黄秋葵（Abelmoschus esculentus（L.）Moench）植物造成不利影响。本研究旨在通过施用有机改良剂来提高黄秋葵植物对盐胁迫诱导的渗透胁迫和氧化胁迫的耐受性。
采用盆栽试验，评估了不同有机改良剂（城市固体废物堆肥、农家肥（FYM）和压榨泥）对盐胁迫（50 mM 氯化钠）下黄秋葵植物的渗透势、水分利用效率、抗氧化酶活性、总可溶性糖、总可溶性脯氨酸、总可溶性蛋白和丙二醛（MDA）含量的影响。有机改良剂的施用量为每盆 5%和 10%，或按不同组合（堆肥+FYM、FYM+压榨泥和堆肥+压榨泥，各为每盆 2.5%和 5%）施用。
与对照相比，盐胁迫下黄秋葵植物的总可溶性糖（60.41%）、总可溶性脯氨酸（33.88%）和 MDA（51%）含量升高，抗氧化酶活性[超氧化物歧化酶（83.54%）、过氧化氢酶（78.61%）、过氧化物酶（53.57%）]增强，表明存在氧化胁迫。与对照相比，盐胁迫显著降低了黄秋葵植物的渗透势（41.78%）和水分利用效率（4.75%）。在盐胁迫条件下，5%（农家肥+压榨泥）是最有效的处理方法，与盐土相比，它显著提高了渗透势（27.05%）、总可溶性糖（4.20%）、总可溶性蛋白（73.62%）和总可溶性脯氨酸（23.20%）含量以及超氧化物歧化酶活性（32.41%）。施用 2.5%（FYM+压榨泥）、5%压榨泥和 10%堆肥分别显著降低了 MDA 含量（27%），并提高了过氧化氢酶（38.64%）和过氧化物酶（48.29%）的活性，从而缓解了黄秋葵植物的氧化胁迫。
使用有机改良剂（城市固体废物堆肥、农家肥和压榨泥）是一种经济有效的方法，可以提高黄秋葵植物对盐胁迫诱导的渗透胁迫和氧化胁迫的耐受性。
