Gröger Anna, Martínez-Albo Ilune, Albà M Mar, Ayté José, Vega Montserrat, Hidalgo Elena
Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/Doctor Aiguader 88, 08003 Barcelona, Spain.
Evolutionary Genomics Group, Research Programme on Biomedical Informatics, Hospital del Mar Research Institute (IMIM), C/Doctor Aiguader 88, 08003 Barcelona, Spain.
Antioxidants (Basel). 2023 Sep 28;12(10):1810. doi: 10.3390/antiox12101810.
Aging is characterized by a number of hallmarks including loss of mitochondrial homeostasis and decay in stress tolerance, among others. Unicellular eukaryotes have been widely used to study chronological aging. As a general trait, calorie restriction and activation of mitochondrial respiration has been proposed to contribute to an elongated lifespan. Most aging-related studies have been conducted with the Crabtree-positive yeasts and , and with deletion collections deriving from these conventional yeast models. We have performed an unbiased characterization of longevity using thirteen fungi species, including and , covering a wide range of the clade. We have determined their mitochondrial activity by oxygen consumption, complex IV activity, and mitochondrial redox potential, and the results derived from these three methodologies are highly overlapping. We have phenotypically compared the lifespans of the thirteen species and their capacity to tolerate oxidative stress. Longevity and elevated tolerance to hydrogen peroxide are correlated in some but not all yeasts. Mitochondrial activity per se cannot anticipate the length of the lifespan. We have classified the strains in four groups, with members of group 1 (, and ) displaying high mitochondrial activity, elevated resistance to oxidative stress, and elongated lifespan.
衰老具有许多特征，包括线粒体稳态丧失和应激耐受性下降等。单细胞真核生物已被广泛用于研究时序衰老。作为一个普遍特征，热量限制和线粒体呼吸的激活被认为有助于延长寿命。大多数与衰老相关的研究是使用克勒勃屈利阳性酵母以及从这些传统酵母模型衍生而来的缺失文库进行的。我们使用了13种真菌物种，包括……，对寿命进行了无偏倚的表征，这些物种涵盖了……进化枝的广泛范围。我们通过氧气消耗、细胞色素c氧化酶活性和线粒体氧化还原电位测定了它们的线粒体活性，并且从这三种方法得出的结果高度重叠。我们从表型上比较了这13个物种的寿命及其耐受氧化应激的能力。在一些但并非所有酵母中，寿命延长与对过氧化氢的耐受性提高相关。线粒体活性本身无法预测寿命的长短。我们将这些菌株分为四组，第1组（……）的成员表现出线粒体活性高、对氧化应激的抗性增强和寿命延长的特点。
