subsp. exhibits probiotic properties in humans. Considering that can be used to study the effects of microorganisms on animal behavior, owing to its simple nervous system, we assessed the impacts of two strains of subsp. -a non-nisin-producing strain, NBRC 100933 (LL100933), and a nisin-producing strain, NBRC 12007 (LL12007)-on the lifespan, locomotion, reproductive capacity of, and lipid accumulation in, . The lifespan of adult fed a mixture (1:1) of OP50 and LL100933 or LL12007 did not show a significant increase compared to that of the group fed a standard diet of OP50. However, the nematodes fed strains showed notable enhancement in their locomotion at all of the tested ages. Further, the beneficial effects of LL100933 and LL12007 were observed in the mutants, but not in the and mutants. The lipid accumulation in the worms of the -fed group was lower than that in the control group at all experimental ages. Overall, LL100933 and LL12007 enhance the locomotor behavior of , likely by modulating the PMK-1/p38 MAPK and SKN-1/Nrf2 transcription factors.