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大肠杆菌中青霉素结合蛋白在加热和去污剂处理后的行为以及青霉素结合蛋白1A和1B的部分纯化

Behavior of penicillin-binding proteins in Escherichia coli upon heat and detergent treatments and partial purification of penicillin-binding proteins 1A and 1B.

作者信息

Matsuzawa H, Datta P, Matsuhashi M

出版信息

J Bacteriol. 1979 Jun;138(3):1029-32. doi: 10.1128/jb.138.3.1029-1032.1979.

DOI:10.1128/jb.138.3.1029-1032.1979
PMID:378927
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC218137/
Abstract

Penicillin-binding proteins differ greatly in heat sensitivity and sensitivity to detergents. The partial purification of penicillin-binding 1A and 1B proteins from Escherichia coli is described.

摘要

青霉素结合蛋白在热敏感性和对去污剂的敏感性方面差异很大。本文描述了从大肠杆菌中部分纯化青霉素结合1A和1B蛋白的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b14/218137/b5bd14ad0b85/jbacter00283-0385-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b14/218137/b5bd14ad0b85/jbacter00283-0385-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b14/218137/b5bd14ad0b85/jbacter00283-0385-a.jpg

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1
Behavior of penicillin-binding proteins in Escherichia coli upon heat and detergent treatments and partial purification of penicillin-binding proteins 1A and 1B.大肠杆菌中青霉素结合蛋白在加热和去污剂处理后的行为以及青霉素结合蛋白1A和1B的部分纯化
J Bacteriol. 1979 Jun;138(3):1029-32. doi: 10.1128/jb.138.3.1029-1032.1979.
2
Improved sensitivity in assays for binding of novel beta-lactam antibiotics to penicillin-binding proteins of Escherichia coli.
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3
Mutants of Escherichia coli which lack a component of penicillin-binding protein 1 are viable.缺乏青霉素结合蛋白1组分的大肠杆菌突变体是有活力的。
FEBS Lett. 1977 Jul 15;79(2):374-8. doi: 10.1016/0014-5793(77)80824-7.
4
Penicillin-binding protein 1B of Escherichia coli exists in dimeric forms.大肠杆菌的青霉素结合蛋白1B以二聚体形式存在。
J Bacteriol. 1991 Sep;173(18):5740-6. doi: 10.1128/jb.173.18.5740-5746.1991.
5
Purification and sequencing of the active site tryptic peptide from penicillin-binding protein 1b of Escherichia coli.大肠杆菌青霉素结合蛋白1b活性位点胰蛋白酶肽段的纯化与测序
Biochemistry. 1985 Jul 2;24(14):3448-53. doi: 10.1021/bi00335a009.
6
Properties of the penicillin-binding proteins of Escherichia coli K12,大肠杆菌K12青霉素结合蛋白的特性
Eur J Biochem. 1977 Jan;72(2):341-52. doi: 10.1111/j.1432-1033.1977.tb11258.x.
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J Antibiot (Tokyo). 1978 Dec;31(12):1283-91. doi: 10.7164/antibiotics.31.1283.
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Mutational evidence for identity of penicillin-binding protein 5 in Escherichia coli with the major D-alanine carboxypeptidase IA activity.大肠杆菌中青霉素结合蛋白5与主要D-丙氨酸羧肽酶IA活性相同的突变证据。
J Bacteriol. 1979 Jan;137(1):644-7. doi: 10.1128/jb.137.1.644-647.1979.
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J Antibiot (Tokyo). 1978 Dec;31(12):1292-5. doi: 10.7164/antibiotics.31.1292.

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本文引用的文献

1
Glycopeptide transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reactions.糖肽转肽酶和D-丙氨酸羧肽酶：对青霉素敏感的酶促反应。
Proc Natl Acad Sci U S A. 1966 Mar;55(3):656-63. doi: 10.1073/pnas.55.3.656.
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Biosynthesis of the peptidoglycan of bacterial cell walls. 8. Peptidoglycan transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reaction in strains of Escherichia coli.细菌细胞壁肽聚糖的生物合成。8. 肽聚糖转肽酶和D-丙氨酸羧肽酶：大肠杆菌菌株中的青霉素敏感酶促反应。
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Penicillin-resistant temperature-sensitive mutants of Escherichia coli which synthesize hypo- or hyper-cross-linked peptidoglycan.
对万古霉素耐药菌株具有活性的万古霉素类似物可抑制细菌转糖基酶而不结合底物。
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Direct quantitation of the number of individual penicillin-binding proteins per cell in Escherichia coli.直接定量大肠杆菌中每个细胞内单个青霉素结合蛋白的数量。
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Differences between inner membrane and peptidoglycan-associated PBP1B dimers of Escherichia coli.大肠杆菌内膜与肽聚糖相关的PBP1B二聚体之间的差异。
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6
Isolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive D-alanyl-D-alanine-cleaving transpeptidase.以青霉素敏感的D-丙氨酰-D-丙氨酸裂解转肽酶形式分离链霉菌K15菌株的膜结合26000道尔顿青霉素结合蛋白。
Biochem J. 1982 Oct 1;207(1):109-15. doi: 10.1042/bj2070109.
7
Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli.负责大肠杆菌杆状形态和美西林敏感性的mrdA和mrdB基因簇。
J Bacteriol. 1980 Jan;141(1):52-7. doi: 10.1128/jb.141.1.52-57.1980.
8
A lacZ-pbpB gene fusion coding for an inducible hybrid protein that recognizes localized sites in the inner membrane of Escherichia coli.一个编码可诱导杂交蛋白的lacZ - pbpB基因融合体，该杂交蛋白可识别大肠杆菌内膜中的定位位点。
J Bacteriol. 1988 Aug;170(8):3333-41. doi: 10.1128/jb.170.8.3333-3341.1988.
9
Dispensability of either penicillin-binding protein-1a or -1b involved in the essential process for cell elongation in Escherichia coli.参与大肠杆菌细胞伸长必需过程的青霉素结合蛋白-1a或-1b的非必需性。
Mol Gen Genet. 1985;200(2):272-7. doi: 10.1007/BF00425435.
10
Cloning and expression of the ponB gene, encoding penicillin-binding protein 1B of Escherichia coli, in heterologous systems.
J Bacteriol. 1990 Aug;172(8):4448-55. doi: 10.1128/jb.172.8.4448-4455.1990.
合成低交联或高交联肽聚糖的大肠杆菌青霉素抗性温度敏感突变体。
J Bacteriol. 1974 Feb;117(2):568-77. doi: 10.1128/jb.117.2.568-577.1974.
4
Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12.参与大肠杆菌K12分裂、伸长和形态形成的不同青霉素结合蛋白。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2999-3003. doi: 10.1073/pnas.72.8.2999.
5
Penicillin-binding proteins and cell shape in E. coli.大肠杆菌中的青霉素结合蛋白与细胞形态
Nature. 1975 Apr 10;254(5500):516-7. doi: 10.1038/254516a0.
6
Mutational evidence for identity of penicillin-binding protein 5 in Escherichia coli with the major D-alanine carboxypeptidase IA activity.大肠杆菌中青霉素结合蛋白5与主要D-丙氨酸羧肽酶IA活性相同的突变证据。
J Bacteriol. 1979 Jan;137(1):644-7. doi: 10.1128/jb.137.1.644-647.1979.
7
Isolation of a mutant of Escherichia coli lacking penicillin-sensitive D-alanine carboxypeptidase IA.缺乏青霉素敏感型D-丙氨酸羧肽酶IA的大肠杆菌突变体的分离
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2631-5. doi: 10.1073/pnas.75.6.2631.
8
On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins.关于大肠杆菌细胞分裂过程：一系列青霉素结合蛋白发生改变的大肠杆菌突变体。
Proc Natl Acad Sci U S A. 1978 Feb;75(2):664-8. doi: 10.1073/pnas.75.2.664.
9
Thermosensitive mutation in Escherichia coli simultaneously causing defects in penicillin-binding protein-1Bs and in enzyme activity for peptidoglycan synthesis in vitro.大肠杆菌中的热敏突变同时导致青霉素结合蛋白-1B的缺陷以及体外肽聚糖合成酶活性的缺陷。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5472-6. doi: 10.1073/pnas.74.12.5472.
10
Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12.在大肠杆菌K12突变体中同时缺失D-丙氨酸羧肽酶IB-C和青霉素结合成分IV。
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2980-4. doi: 10.1073/pnas.74.7.2980.