Ahn Chae-Ryeong, Baek Seung-Ho
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
College of Korean Medicine, Dongguk University, 32 Dongguk-ro, Goyang-si 10326, Republic of Korea.
Biomedicines. 2023 Oct 5;11(10):2710. doi: 10.3390/biomedicines11102710.
Gastric cancer remains a global health threat, particularly in Asian countries. Current treatment methods include surgery, chemotherapy, and radiation therapy. However, they all have limitations, such as adverse side effects, tumor resistance, and patient tolerance. Hyperthermia therapy uses heat to selectively target and destroy cancer cells, but it has limited efficacy when used alone. Linderae Radix (LR), a natural compound with thermogenic effects, has the potential to enhance the therapeutic efficacy of hyperthermia treatment. In this study, we investigated the synergistic anticancer effects of cotreatment with LR and 43 °C hyperthermia in AGS gastric cancer cells. The cotreatment inhibited AGS cell proliferation, induced apoptosis, caused cell cycle arrest, suppressed heat-induced heat shock responses, increased reactive oxygen species (ROS) generation, and promoted mitogen-activated protein kinase phosphorylation. N-acetylcysteine pretreatment abolished the apoptotic effect of LR and hyperthermia cotreatment, indicating the crucial role of ROS in mediating the observed anticancer effects. These findings highlight the potential of LR as an adjuvant to hyperthermia therapy for gastric cancer. Further research is needed to validate these findings in vivo, explore the underlying molecular pathways, and optimize treatment protocols for the development of novel and effective therapeutic strategies for patients with gastric cancer.
胃癌仍然是一个全球性的健康威胁，尤其是在亚洲国家。目前的治疗方法包括手术、化疗和放疗。然而，它们都有局限性，如副作用、肿瘤耐药性和患者耐受性。热疗利用热量选择性地靶向并破坏癌细胞，但单独使用时疗效有限。具有产热作用的天然化合物乌药（LR）有可能增强热疗的治疗效果。在本研究中，我们研究了LR与43℃热疗联合处理对AGS胃癌细胞的协同抗癌作用。联合处理抑制了AGS细胞增殖，诱导了细胞凋亡，导致细胞周期停滞，抑制了热诱导的热休克反应，增加了活性氧（ROS）的产生，并促进了丝裂原活化蛋白激酶磷酸化。N-乙酰半胱氨酸预处理消除了LR与热疗联合处理的凋亡作用，表明ROS在介导观察到的抗癌作用中起关键作用。这些发现突出了LR作为胃癌热疗辅助剂的潜力。需要进一步的研究来在体内验证这些发现，探索潜在的分子途径，并优化治疗方案，以开发针对胃癌患者的新型有效治疗策略。
