Lee Myeong Joon, Cho Yeonoh, Hwang Yujin, Jo Youngheun, Kim Yeon-Gu, Lee Seung Hwan, Lee Jong Hun
Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea.
Department of Urology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Foods. 2023 Oct 20;12(20):3836. doi: 10.3390/foods12203836.
Common prostate diseases such as prostatitis and benign prostatic hyperplasia (BPH) have a high incidence at any age. Cellular stresses, such as reactive oxygen species (ROS) and chronic inflammation, are implicated in prostate enlargement and cancer progression and development. Kaempferol is a flavonoid found in abundance in various plants, including broccoli and spinach, and has been reported to exhibit positive biological activities, such as antioxidant and anti-inflammatory properties. In the present study, we introduced prostate organoids to investigate the protective effects of kaempferol against various cellular stresses. The levels of COX-2, iNOS, p-IκB, a pro-inflammatory cytokine, and ROS were increased by LPS treatment but reversed by kaempferol treatment. Kaempferol activated the nuclear factor erythroid 2-related factor 2(Nrf2)-related pathway and enhanced the mitochondrial quality control proteins PGC-1α, PINK1, Parkin, and Beclin. The increase in mitochondrial ROS and oxygen consumption induced by LPS was stabilized by kaempferol treatment. First, our study used prostate organoids as a novel evaluation platform. Secondly, it was demonstrated that kaempferol could alleviate the mitochondrial damage in LPS-induced induced prostate organoids by reducing the production of mitochondrial ROS.
前列腺炎和良性前列腺增生（BPH）等常见前列腺疾病在任何年龄段的发病率都很高。细胞应激，如活性氧（ROS）和慢性炎症，与前列腺肿大以及癌症的进展和发展有关。山奈酚是一种在包括西兰花和菠菜在内的各种植物中大量存在的类黄酮，据报道具有抗氧化和抗炎等积极的生物学活性。在本研究中，我们引入前列腺类器官来研究山奈酚对各种细胞应激的保护作用。脂多糖（LPS）处理会使COX-2、诱导型一氧化氮合酶（iNOS）、磷酸化核因子κB抑制蛋白（p-IκB）（一种促炎细胞因子）和ROS的水平升高，但山奈酚处理可使其逆转。山奈酚激活了核因子红细胞2相关因子2（Nrf2）相关通路，并增强了线粒体质量控制蛋白过氧化物酶体增殖物激活受体γ辅激活因子1α（PGC-1α）、帕金森病相关蛋白1（PINK1）、帕金蛋白（Parkin）和贝林蛋白（Beclin）。山奈酚处理可稳定LPS诱导的线粒体ROS增加和氧气消耗。首先，我们的研究将前列腺类器官用作一种新型评估平台。其次，研究表明山奈酚可通过减少线粒体ROS的产生来减轻LPS诱导的前列腺类器官中的线粒体损伤。
