Lee Jeongeun, Belal Shah Ahmed, Lin Xi, Park Jinryong, Shim Kwanseob
Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Department of Animal Biotechnology, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Animals (Basel). 2023 Oct 14;13(20):3209. doi: 10.3390/ani13203209.
Heat stress inhibits cell proliferation as well as animal production. Here, we aimed to demonstrate that 9-mer disulfide dimer peptide (CopA3) supplementation stabilizes porcine muscle satellite cell (PMSC) proliferation and heat shock protein (HSP) expression at different temperatures. Therefore, we investigated the beneficial effects of CopA3 on PMSCs at three different temperatures (37, 39, and 41 °C). Based on temperature and CopA3 treatment, PMSCs were divided into six different groups including treatment and control groups for each temperature. Cell viability was highest with 10 µg/mL CopA3 and decreased as the concentration increased in a dose-dependent manner. CopA3 significantly increased the cell viability at all temperatures at 24 and 48 h. It significantly decreased apoptosis compared to that in the untreated groups. In addition, it decreased the apoptosis-related protein, Bcl-2-associated X (BAX), expression at 41 °C. Notably, temperature and CopA3 had no effects on the apoptosis-related protein, caspase 3. Expression levels of HSP40, HSP70, and HSP90 were significantly upregulated, whereas those of HSP47 and HSP60 were not affected by temperature changes. Except HSP90, CopA3 did not cause temperature-dependent changes in protein expression. Therefore, CopA3 promotes cell proliferation, inhibits apoptosis, and maintains stable HSP expression, thereby enhancing the heat-stress-tolerance capacity of PMSCs.
热应激会抑制细胞增殖以及动物生产。在此，我们旨在证明补充9聚体二硫键二聚体肽（CopA3）可在不同温度下稳定猪肌肉卫星细胞（PMSC）的增殖和热休克蛋白（HSP）表达。因此，我们研究了CopA3在三种不同温度（37、39和41°C）下对PMSC的有益作用。根据温度和CopA3处理，PMSC被分为六个不同的组，每个温度下都有处理组和对照组。10 µg/mL CopA3时细胞活力最高，且随着浓度增加以剂量依赖性方式下降。CopA3在所有温度下24小时和48小时时均显著提高细胞活力。与未处理组相比，它显著降低了细胞凋亡。此外，在41°C时它降低了凋亡相关蛋白Bcl-2相关X蛋白（BAX）的表达。值得注意的是，温度和CopA3对凋亡相关蛋白caspase 3没有影响。HSP40、HSP70和HSP90的表达水平显著上调，而HSP47和HSP60的表达水平不受温度变化影响。除HSP90外，CopA3不会导致蛋白质表达出现温度依赖性变化。因此，CopA3促进细胞增殖，抑制细胞凋亡，并维持稳定的HSP表达，从而增强PMSC的热应激耐受能力。
