Feng Wenping, Zhao Zeping, Wang Jiteng, Han Tao
Fishery College, Zhejiang Ocean University, 1 Haida South Road Changzhi Island Lincheng New Area, Zhoushan 316022, China.
Animals (Basel). 2023 Oct 15;13(20):3220. doi: 10.3390/ani13203220.
The swimming crab is one of the most important economic species in China and its mature ovary often determines its commercial value and production. Although the ovary maturation of crustaceans is generally affected by exogenous nutrition, the specific nutritional needs of ovary maturation of are poorly understood. To this end, we collected the samples with five ovarian maturation stages and measured their biochemical composition of the ovary, hepatopancreas, and muscle at each ovarian developmental stage. We further analyzed their relation to the ovarian developmental stage of by principal components analysis (PCA). We found the levels of branched-chain amino acids, long-chain polyunsaturated fatty acids (LC-PUFA), and monounsaturated fatty acids (MUFAs) in the ovary and hepatopancreas increased during the ovary maturation process, and also passively correlated with ovarian developmental stage, which highlights the necessity of these specific nutrients for oogenesis and for improving the nutrient quality of crabs. In addition, we found an increasing tendency of carotenoid content and phosphatidylcholine in phospholipid in the ovary from the pre-developmental stage to the proliferative stage, but not in the hepatopancreas and muscle, which highlights the possible involvement of carotenoids during the rapid oocyte development process. Our study may provide valuable information for developing a suitable broodstock diet that promotes the ovarian maturation of adult and ensures high-quality larval production.
梭子蟹是中国最重要的经济物种之一，其成熟卵巢往往决定了它的商业价值和产量。虽然甲壳类动物的卵巢成熟通常受外源营养影响，但关于梭子蟹卵巢成熟的具体营养需求却知之甚少。为此，我们收集了处于五个卵巢成熟阶段的样本，并测量了每个卵巢发育阶段其卵巢、肝胰腺和肌肉的生化组成。我们通过主成分分析（PCA）进一步分析了它们与梭子蟹卵巢发育阶段的关系。我们发现，在卵巢成熟过程中，卵巢和肝胰腺中支链氨基酸、长链多不饱和脂肪酸（LC-PUFA）和单不饱和脂肪酸（MUFA）的水平升高，并且与卵巢发育阶段呈正相关，这突出了这些特定营养素对于卵子发生和提高蟹类营养品质的必要性。此外，我们发现从发育前期到增殖期，卵巢中类胡萝卜素含量和磷脂中磷脂酰胆碱呈上升趋势，但在肝胰腺和肌肉中并非如此，这突出了类胡萝卜素在卵母细胞快速发育过程中可能发挥的作用。我们的研究可能为制定合适的亲鱼饲料提供有价值的信息，这种饲料可促进成年梭子蟹的卵巢成熟并确保高质量的幼体生产。
