College of Ocean and Earth Sciences, Xiamen University, Xiamen, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Gen Comp Endocrinol. 2019 Sep 1;280:115-122. doi: 10.1016/j.ygcen.2019.04.019. Epub 2019 Apr 16.
Estradiol is an important sex steroid hormone that involved in regulation of animal lipid metabolism. However, the effect of estradiol on lipid metabolism in swimming crab (Portunus trituberculatus) is unclear. The present study investigated the effect of four concentrations of exogenous estradiol (0, 0.01, 0.1 and 1 μg g crab weight) on the expression levels of lipid metabolism-related genes, lipid composition and histology of hepatopancreas in the P. trituberculatus. The results showed that the mRNA levels of carnitine palmitoyltransferase I and II (CPT-I and CPT-II) increased significantly at the low concentrations (0.01 μg g and 0.1 μg g), while decreased significantly in the highest concentration (1 μg g). The mRNA levels of acyl-CoA oxidase (ACOX), fatty acid transport protein (FATP), fatty acid-binding protein (FABP), diacylglycerol acyltransferase 1 (DGAT1) and acetyl-CoA carboxylase (ACC) were significantly down-regulated. The transcripts of fatty acid synthase (FAS) and fatty acyl desaturase (FAD) decreased significantly only in 1 μg g treatment. All estradiol treatments (0.01, 0.1 and 1 μg g) had significantly higher percentages of 20:4n6, 20:5n3 and 22:6n3, but lower percentages of total monounsaturated fatty acids and polar lipids than the control treatment (0 μg g). Histological observations indicated the size of B cell became larger under estradiol treatment. The results indicated that estradiol promoted lipid catabolism in the hepatopancreas of P. trituberculatus.
雌二醇是一种重要的性激素，参与动物脂质代谢的调节。然而，雌二醇对三疣梭子蟹（Portunus trituberculatus）脂质代谢的影响尚不清楚。本研究探讨了四种外源性雌二醇浓度（0、0.01、0.1 和 1μg/g 蟹体重）对三疣梭子蟹肝胰腺脂质代谢相关基因表达水平、脂质组成和组织学的影响。结果表明，低浓度（0.01μg/g 和 0.1μg/g）时，肉碱棕榈酰转移酶 I 和 II（CPT-I 和 CPT-II）的 mRNA 水平显著升高，而在最高浓度（1μg/g）时显著降低。酰基辅酶 A 氧化酶（ACOX）、脂肪酸转运蛋白（FATP）、脂肪酸结合蛋白（FABP）、二酰基甘油酰基转移酶 1（DGAT1）和乙酰辅酶 A 羧化酶（ACC）的 mRNA 水平均显著下调。脂肪酸合成酶（FAS）和脂肪酸去饱和酶（FAD）的转录物仅在 1μg/g 处理时显著降低。所有雌二醇处理（0.01、0.1 和 1μg/g）的 20:4n6、20:5n3 和 22:6n3 百分比显著升高，但总单不饱和脂肪酸和极性脂质的百分比显著低于对照处理（0μg/g）。组织学观察表明，B 细胞在雌二醇处理下体积变大。结果表明，雌二醇促进了三疣梭子蟹肝胰腺的脂质分解代谢。
