Snider Alexandria P, Gomes Renata S, Summers Adam F, Tenley Sarah C, Abedal-Majed Mohamed A, McFee Renee M, Wood Jennifer R, Davis John S, Cupp Andrea S
United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA.
Department of Animal Science, University of Nebraska-Lincoln, 3940 Fair Street, Lincoln, NE 68583, USA.
Animals (Basel). 2023 Oct 21;13(20):3289. doi: 10.3390/ani13203289.
The process of follicle maturation leading to ovulation is a key milestone in female fertility. It is known that circulating lipids and cytokines play a role in the follicle's ability to go through follicular maturation and the ovulatory processes. However, the specific mechanisms are not well understood. We posit that dysregulation of granulosa cells influences the ovarian environment, which tries to adapt by changing released lipids and cytokines to achieve follicular maturation. Eleven non-lactating adult females underwent estrus synchronization with two injections of PGF2α 14 days apart. Daily blood samples were collected for 28 days to monitor steroid hormone production after the second injection. To understand the potential impacts of lipids and cytokines during ovulation, a low-dose FSH stimulation (FSHLow) was performed after resynchronization of cows, and daily blood samples were collected for 14 days to monitor steroid hormone production until ovariectomies. The lipidomic analysis demonstrated increased circulating diacylglycerides and triacylglycerides during the mid-luteal phase and after FSHLow treatment. Cholesteryl esters decreased in circulation but increased in follicular fluid (FF) after FSHLow. Increased circulating concentrations of TNFα and reduced CXCL9 were observed in response to FSHLow. Therefore, specific circulating lipids and cytokines may serve as markers of normal follicle maturation.
卵泡成熟并导致排卵的过程是女性生育能力的一个关键里程碑。已知循环脂质和细胞因子在卵泡经历卵泡成熟和排卵过程的能力中发挥作用。然而，具体机制尚不清楚。我们推测，颗粒细胞的失调会影响卵巢环境，卵巢环境试图通过改变释放的脂质和细胞因子来实现卵泡成熟。11只非泌乳成年雌性动物每隔14天注射两次PGF2α进行发情同步。在第二次注射后每天采集血样28天，以监测类固醇激素的产生。为了了解排卵期间脂质和细胞因子的潜在影响，在奶牛重新同步后进行低剂量FSH刺激（FSHLow），并每天采集血样14天，以监测类固醇激素的产生，直至进行卵巢切除术。脂质组学分析表明，在黄体中期和FSHLow治疗后，循环中的二酰甘油和三酰甘油增加。FSHLow后，胆固醇酯在循环中减少，但在卵泡液（FF）中增加。对FSHLow的反应观察到循环中TNFα浓度增加，CXCL9减少。因此，特定的循环脂质和细胞因子可能作为正常卵泡成熟的标志物。
