Cui Siqi, Du Jie, Zhu Lin, Xin Di, Xin Yuhua, Zhang Jianli
Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Microorganisms. 2023 Oct 9;11(10):2517. doi: 10.3390/microorganisms11102517.
To assess the diversity of bacterial taxa in Antarctic soils and obtain novel microbial resources, 15 samples from 3 sampling sites (DIS5, GWS7, FPS10) of South Shetland Islands and 2 sampling sites (APS18, CIS17) of Antarctic Peninsula were collected. High-throughput sequencing (HTS) of 16S rRNA genes within these samples was conducted on an Illumina Miseq platform. A total of 140,303 16S rRNA gene reads comprising 802 operational taxonomic units (OTUs) were obtained. After taxonomic classification, 25 phyla, 196 genera, and a high proportion of unidentified taxa were detected, among which seven phyla and 99 genera were firstly detected in Antarctica. The bacterial communities were dominated by (40.40%), (17.14%), (10.55%) and (10.26%). Based on the HTS analyses, cultivation-dependent techniques were optimized to identify the cultivable members. A total of 30 different genera including 91 strains were obtained, the majority of which has previously been reported from Antarctica. However, for the genera , , , , , and , this is the first report in Antarctica. In addition, seven strains represented novel taxa, two of which were psychropoilic and could be valuable resources for further research of cold-adaptability and their ecological significance in Antarctica.
为评估南极土壤中细菌类群的多样性并获取新的微生物资源，采集了南设得兰群岛3个采样点（DIS5、GWS7、FPS10）和南极半岛2个采样点（APS18、CIS17）的15个样本。在Illumina Miseq平台上对这些样本中的16S rRNA基因进行了高通量测序（HTS）。共获得140,303条16S rRNA基因读数，包含802个操作分类单元（OTU）。经过分类学分类，检测到25个门、196个属以及高比例的未鉴定类群，其中7个门和99个属是首次在南极洲检测到。细菌群落主要由（40.40%）、（17.14%）、（10.55%）和（10.26%）主导。基于高通量测序分析，优化了依赖培养的技术以鉴定可培养成员。共获得包括91株菌株在内的30个不同属，其中大多数此前已在南极洲报道过。然而，对于属、、、、、和，这是在南极洲的首次报道。此外，7株菌株代表新的类群，其中2株是嗜冷菌，可能是进一步研究南极洲冷适应性及其生态意义的宝贵资源。
