与医院环境中三个临床组（炎症性肠病、感染和医护人员）携带情况相关的肠道微生物群

Gut Microbiota Associated with Carriage in Three Clinical Groups (Inflammatory Bowel Disease, Infection and Healthcare Workers) in Hospital Field.

作者信息

Martinez Elisa, Crevecoeur Sebastien, Thirion Carine, Grandjean Jessica, Fall Papa Abdoulaye, Hayette Marie-Pierre, Michel Moutschen, Taminiau Bernard, Louis Edouard, Daube Georges

机构信息

Food Microbiology Lab, Fundamental and Applied Research for Animals and Health (FARAH), Department of Food Sciences, Faculty of Veterinary Medicine, University of Liege, 4000 Liège, Belgium.

Department of Clinical Sciences, Immunopathology-Infectious Diseases and General Internal Medicine, University Hospital CHU of Liege, 4000 Liège, Belgium.

出版信息

Microorganisms. 2023 Oct 10;11(10):2527. doi: 10.3390/microorganisms11102527.

DOI:10.3390/microorganisms11102527

PMID:37894185

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10609531/

Abstract

is an anaerobic spore-forming Gram-positive bacterium. carriage and 16S rDNA profiling were studied in three clinical groups at three different sampling times: inflammatory bowel disease (IBD) patients, infection (CDI) patients and healthcare workers (HCWs). Diversity analysis was realized in the three clinical groups, the positive and negative carriage groups and the three analysis periods. Concerning the three clinical groups, β-diversity tests showed significant differences between them, especially between the HCW group and IBD group and between IBD patients and CDI patients. The Simpson index (evenness) showed a significant difference between two clinical groups (HCWs and IBD). Several genera were significantly different in the IBD patient group (, ) and in the CDI patient group (, ). Concerning the positive and negative carriage groups, β-diversity tests showed significant differences. Shannon, Simpson and InvSimpson indexes showed significant differences between the two groups. Several genera had significantly different relative prevalences in the negative group (, , , ) and the positive group (, _ge and _ge). A microbiota footprint was detected in -positive carriers. More experiments are needed to test this microbiota footprint to see its impact on infection.

摘要

是一种厌氧产芽孢革兰氏阳性菌。在三个不同采样时间对三个临床组进行了携带情况和16S rDNA分析：炎症性肠病（IBD）患者、艰难梭菌感染（CDI）患者和医护人员（HCWs）。在三个临床组、阳性和阴性携带组以及三个分析时间段内进行了多样性分析。关于三个临床组，β多样性测试显示它们之间存在显著差异，特别是医护人员组与IBD组之间以及IBD患者与CDI患者之间。辛普森指数（均匀度）显示两个临床组（医护人员和IBD）之间存在显著差异。在IBD患者组（，）和CDI患者组（，）中，有几个属存在显著差异。关于阳性和阴性携带组，β多样性测试显示存在显著差异。香农、辛普森和反辛普森指数在两组之间显示出显著差异。在阴性组（，，，）和阳性组（，_ge和_ge）中，有几个属的相对患病率存在显著差异。在艰难梭菌阳性携带者中检测到了微生物群特征。需要进行更多实验来测试这种微生物群特征，以观察其对艰难梭菌感染的影响。