Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea.
Institute for Liver and Digestive Diseases, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of Korea.
Molecules. 2023 Oct 10;28(20):7007. doi: 10.3390/molecules28207007.
Lowering blood cholesterol levels is crucial for reducing the risk of cardiovascular disease in patients with familial hypercholesterolemia. To develop (L.) Britt. leaves as a functional food with a cholesterol-lowering effect, in this study, we collected (L.) Britt. leaves from different regions of China and Republic of Korea. On the basis of the extraction yield (all components; g/kg), we selected (L.) Britt. leaves from Hebei Province, China with an extract yield of 60.9 g/kg. After evaluating different concentrations of ethanol/water solvent for (L.) Britt. leaves, with luteolin 7-glucuronide as the indicator component, we selected a 30% ethanol/water solvent with a high luteolin 7-glucuronide content of 0.548 mg/g in (L.) Britt. leaves. Subsequently, we evaluated the cholesterol-lowering effects of (L.) Britt. leaf extract and luteolin 7-glucuronide by detecting total cholesterol in HepG2 cells. The 30% ethanol extract lowered cholesterol levels significantly by downregulating 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase expression. This suggests that (L.) Britt leaves have significant health benefits and can be explored as a potentially promising food additive for the prevention of hypercholesterolemia-related diseases.
降低血液胆固醇水平对于降低家族性高胆固醇血症患者患心血管疾病的风险至关重要。为了开发具有降胆固醇作用的（L.） Britt. 叶功能性食品，本研究从中国和韩国的不同地区收集了（L.） Britt. 叶。基于提取物得率（所有成分；g/kg），我们选择了中国河北省的（L.） Britt. 叶，其提取物得率为 60.9 g/kg。在评估不同浓度的乙醇/水溶液对（L.） Britt. 叶后，以木犀草素 7-葡萄糖醛酸苷为指标成分，我们选择了一种 30%乙醇/水溶液，其中（L.） Britt. 叶中木犀草素 7-葡萄糖醛酸苷的含量高达 0.548 mg/g。随后，我们通过检测 HepG2 细胞中的总胆固醇来评估（L.） Britt. 叶提取物和木犀草素 7-葡萄糖醛酸苷的降胆固醇作用。30%乙醇提取物通过下调 3-羟基-3-甲基戊二酰辅酶 A 还原酶的表达显著降低胆固醇水平。这表明（L.） Britt. 叶具有显著的健康益处，可以作为预防与高胆固醇血症相关疾病的潜在有前途的食品添加剂进行探索。
