Clinic for Digestive Surgery-First Surgical Clinic, University Clinical Center of Serbia, 11000 Belgrade, Serbia.
Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia.
Int J Mol Sci. 2023 Oct 11;24(20):15083. doi: 10.3390/ijms242015083.
The Schlafen 11 (SLFN11) protein has recently emerged as pivotal in DNA damage conditions, with predictive potential for tumor response to cytotoxic chemotherapies. Recent discoveries also showed that the programmed death ligand 1 (PD-L1) protein can be found on malignant cells, providing an immune evasion mechanism exploited by different tumors. Additionally, excessive generation of free radicals, redox imbalance, and consequential DNA damage can affect intestinal cell homeostasis and lead to neoplastic transformation. Therefore, our study aimed to investigate the significance of SLFN11 and PD-L1 proteins and redox status parameters as prognostic biomarkers in CRC patients. This study included a total of 155 CRC patients. SLFN11 and PD-L1 serum levels were measured with ELISA and evaluated based on redox status parameters, sociodemographic and clinical characteristics, and survival. The following redox status parameters were investigated: spectrophotometrically measured superoxide dismutase (SOD), sulfhydryl (SH) groups, advanced oxidation protein products (AOPP), malondialdehyde (MDA), pro-oxidant-antioxidant balance (PAB), and superoxide anion (O). The prooxidative score, antioxidative score, and OXY-SCORE were also calculated. The results showed significantly shorter survival in patients with higher OXY-SCOREs and higher levels of serum SLFN11, while only histopathology-analysis-related factors showed significant prognostic value. OXY-SCORE and SLFN11 levels may harbor prognostic potential in CRC patients.
SLFN11 蛋白在 DNA 损伤条件下最近被认为是至关重要的，对肿瘤对细胞毒化疗的反应具有预测潜力。最近的发现还表明，程序性死亡配体 1（PD-L1）蛋白可以在恶性细胞上找到，为不同的肿瘤提供了一种免疫逃避机制。此外，自由基的过度产生、氧化还原失衡以及随之而来的 DNA 损伤会影响肠道细胞的动态平衡，并导致肿瘤转化。因此，我们的研究旨在探讨 SLFN11 和 PD-L1 蛋白以及氧化还原状态参数作为 CRC 患者预后生物标志物的意义。这项研究共纳入了 155 名 CRC 患者。我们使用 ELISA 法测量了 SLFN11 和 PD-L1 的血清水平，并根据氧化还原状态参数、社会人口学和临床特征以及生存情况进行了评估。我们研究了以下氧化还原状态参数：分光光度法测量的超氧化物歧化酶（SOD）、巯基（SH）基团、高级氧化蛋白产物（AOPP）、丙二醛（MDA）、促氧化剂-抗氧化剂平衡（PAB）和超氧阴离子（O）。还计算了促氧化剂评分、抗氧化剂评分和 OXY-SCORE。结果显示，OXY-SCORE 较高和血清 SLFN11 水平较高的患者生存时间明显缩短，而只有组织病理学分析相关因素显示出显著的预后价值。OXY-SCORE 和 SLFN11 水平可能具有 CRC 患者的预后潜力。
