Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de Mexico C.P. 07360, Mexico.
Coordinación de la Investigación Científica, Red de Apoyo a la Investigación, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de Mexico C.P. 14080, Mexico.
Int J Mol Sci. 2023 Oct 14;24(20):15193. doi: 10.3390/ijms242015193.
Among malignant neoplasms, pancreatic ductal adenocarcinoma (PDAC) has one of the highest fatality rates due to its late detection. Therefore, it is essential to discover a noninvasive, early, specific, and sensitive diagnostic method. MicroRNAs (miRNAs) are attractive biomarkers because they are accessible, highly specific, and sensitive. It is crucial to find miRNAs that could be used as possible biomarkers because PDAC is the eighth most common cause of cancer death in Mexico. With the help of microRNA microarrays, differentially expressed miRNAs (DEmiRNAs) were found in PDAC tissues. The presence of these DEmiRNAs in the plasma of Mexican patients with PDAC was determined using RT-qPCR. Receiver operating characteristic curve analysis was performed to determine the diagnostic capacity of these DEmiRNAs. Gene Expression Omnibus datasets (GEO) were employed to verify our results. The Prisma V8 statistical analysis program was used. Four DEmiRNAs in plasma from PDAC patients and microarray tissues were found. Serum samples from patients with PDAC were used to validate their overexpression in GEO databases. We discovered a new panel of the two miRNAs miR-222-3p and miR-221-3p that could be used to diagnose PDAC, and when miR-221-3p and miR-222-3p were overexpressed, survival rates decreased. Therefore, miR-222-3p and miR-221-3p might be employed as noninvasive indicators for the diagnosis and survival of PDAC in Mexican patients.
在恶性肿瘤中，由于胰腺导管腺癌 (PDAC) 发现较晚，其死亡率位居榜首。因此，发现一种非侵入性、早期、特异性和敏感性的诊断方法至关重要。microRNAs (miRNAs) 作为有吸引力的生物标志物，因为它们具有易得性、高度特异性和敏感性。找到可作为潜在生物标志物的 miRNAs 非常重要，因为 PDAC 是墨西哥第八大常见癌症死因。借助 microRNA 微阵列，在 PDAC 组织中发现了差异表达的 miRNAs (DEmiRNAs)。使用 RT-qPCR 测定了这些 DEmiRNAs 在墨西哥 PDAC 患者血浆中的存在。进行了接收器工作特征曲线分析以确定这些 DEmiRNAs 的诊断能力。使用基因表达综合数据库 (GEO) 来验证我们的结果。使用 Prisma V8 统计分析程序。在 PDAC 患者的血浆中发现了 4 种 DEmiRNAs 及其微阵列组织。使用来自 PDAC 患者的血清样本在 GEO 数据库中验证了它们的过表达。我们发现了一个新的由两个 miRNA miR-222-3p 和 miR-221-3p 组成的小组，可以用于诊断 PDAC，并且当 miR-221-3p 和 miR-222-3p 过表达时，生存率降低。因此，miR-222-3p 和 miR-221-3p 可能被用作墨西哥 PDAC 患者诊断和生存的非侵入性指标。
