Gamble H R, Murrell K D, Marti H P
Am J Vet Res. 1986 Nov;47(11):2396-9.
Pigs were inoculated with Trichinella spiralis excretory-secretory products derived from short-term in vitro maintenance of infective muscle larvae. Intraperitoneal administration of excretory-secretory products in Freund's complete adjuvant or aluminum hydroxide induced moderate, but variable, degrees of immunity to challenge exposure in a nondose-dependent manner; IM administration of products in Freund's incomplete adjuvant was less successful. Inoculated pigs harbored fewer adult worms, and the fecundity of female worms (numbers of newborn larvae shed in vitro) recovered after challenge exposure was significantly lower (alpha = 0.05) than the fecundity of females recovered from control pigs. The degree of resistance in inoculated pigs was directly related to serotiter against excretory-secretory antigens, as determined in an enzyme-linked immunosorbent assay.
用旋毛虫感染性肌幼虫短期体外培养产生的排泄分泌产物接种猪。在弗氏完全佐剂或氢氧化铝中腹腔注射排泄分泌产物以非剂量依赖方式诱导了对攻击感染的中等但可变程度的免疫；在弗氏不完全佐剂中肌肉注射产物的效果较差。接种的猪体内成虫数量较少，攻击感染后恢复的雌虫繁殖力（体外排出的新生幼虫数量）显著低于从对照猪中恢复的雌虫繁殖力（α = 0.05）。通过酶联免疫吸附测定确定，接种猪的抗性程度与针对排泄分泌抗原的血清滴度直接相关。
