Research and Development Department, Davos Life Science, 3 Biopolis Drive, #04-19 Synapse, Singapore 138623, Singapore.
Research and Development Department, KL-Kepong Oleomas (KLK Oleo), Level 8, Menara KLK, No 1, Jalan PJU 7/6, Mutiara Damansara, Petaling Jaya 47810, Malaysia.
Int J Mol Sci. 2023 Oct 19;24(20):15373. doi: 10.3390/ijms242015373.
Our skin is constantly exposed to blue light (BL), which is abundant in sunlight and emitted by digital devices. Prolonged exposure to BL can lead to oxidative stress-induced damages and skin hyperpigmentation. For this study, we used a cell line-based model to examine the protective effects of tocotrienol-rich fraction (TRF) on BL-induced oxidative stress and hyperpigmentation in B16-F1 melanocytes. Alpha-tocopherol (αTP) was used as a comparator. Molecular assays such as cell viability assay, flow cytometry, western blotting, fluorescence imaging, melanin and tyrosinase analysis were performed. Our results showed that TRF effectively suppressed the formation of reactive oxygen species and preserved the mitochondrial membrane potential. Additionally, TRF exhibited anti-apoptotic properties by reducing the activation of the p38 mitogen-activated protein kinase molecule and downregulating the expression of cleaved caspase-3. Moreover, TRF modulated tyrosinase activity, resulting in a lowered rate of melanogenesis and reduced melanin production. In contrast, αTP did not exhibit significant protective effects against skin damages and pigmentation in BL-induced B16-F1 cells. Therefore, this study indicates that TRF may offer superior protective effects over αTP against the effects of BL on melanocytes. These findings demonstrate the potential of TRF as a protective natural ingredient that acts against BL-induced skin damages and hyperpigmentation via its anti-oxidative and anti-melanogenic properties.
我们的皮肤会持续受到蓝光（BL）的照射，这种光线在阳光中大量存在，并且也会由数字设备发出。长时间接触 BL 会导致氧化应激引起的损伤和皮肤色素沉着过度。在这项研究中，我们使用细胞系模型来研究生育三烯酚丰富部分（TRF）对 BL 诱导的 B16-F1 黑素细胞氧化应激和色素沉着过度的保护作用。α-生育酚（αTP）被用作比较物。进行了细胞活力测定、流式细胞术、Western blot、荧光成像、黑色素和酪氨酸酶分析等分子测定。我们的结果表明，TRF 能有效抑制活性氧的形成并维持线粒体膜电位。此外，TRF 通过减少 p38 丝裂原活化蛋白激酶分子的激活和下调裂解半胱天冬酶-3 的表达，表现出抗凋亡作用。此外，TRF 调节酪氨酸酶活性，导致黑色素生成率降低和黑色素生成减少。相比之下，αTP 对 BL 诱导的 B16-F1 细胞中的皮肤损伤和色素沉着没有显著的保护作用。因此，这项研究表明，TRF 可能比 αTP 对黑素细胞的 BL 效应具有更好的保护作用。这些发现表明，TRF 可能作为一种天然的保护成分，通过其抗氧化和抗黑色素生成特性，对抗 BL 引起的皮肤损伤和色素沉着过度。
