中国大丽花普通花叶病毒分离物的鉴定与基因组特征
Identification and Genome Characterization of a Dahlia Common Mosaic Virus Isolate from China.
机构信息
College of Plant Protection, Northeast Agricultural University, Harbin 150030, China.
College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
出版信息
Genes (Basel). 2023 Sep 22;14(10):1833. doi: 10.3390/genes14101833.
Dahlia () is a widely cultivated ornamental and medicinal plant in China. Recently, dahlia plants with symptoms of leaf mottling and distortion were collected in Hohhot, Inner Mongolia, China. The presence of dahlia common mosaic virus (DCMV), an unassigned species in the genus , was confirmed by high-throughput sequencing. Three fragments of DCMV Inner Mongolia isolate (DCMV-IN) were PCR-amplified with specific primers, sequenced and assembled into the complete genome sequence with a GenBank accession number of OR494328. The double-stranded circular DNA genome of DCMV-IN consists of 7949 bp and contains six open reading frames (ORFs). Sequence analysis showed that DCMV-IN shared high sequence identities with other DCMV isolates available in the GenBank database. Phylogenetic analysis of DCMV isolates and other representative caulimoviruses based on genome sequence clustered four DCMV isolates to a single branch which was closest to dahlia mosaic virus (DMV). No recombination event was detected among the four DCMV isolates.
大丽花在中国是一种广泛种植的观赏和药用植物。最近，在中国内蒙古呼和浩特收集到了出现叶片斑驳和畸形症状的大丽花植株。高通量测序证实存在大丽花普通花叶病毒（DCMV），这是 属中的一个未分配种。用特异性引物通过 PCR 扩增出大丽花内蒙古分离株（DCMV-IN）的三个片段，测序并组装成完整的基因组序列，GenBank 登录号为 OR494328。DCMV-IN 的双链环状 DNA 基因组由 7949 bp 组成，包含六个开放阅读框（ORFs）。序列分析表明，DCMV-IN 与 GenBank 数据库中其他可用的 DCMV 分离株具有很高的序列同一性。基于基因组序列对 DCMV 分离株和其他代表性 caulimoviruses 的系统发育分析将四个 DCMV 分离株聚类到一个与大丽花花叶病毒（DMV）最接近的单独分支上。在这四个 DCMV 分离株之间未检测到重组事件。
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