School of Life Science and Bioengineering, Jining University, Jining 273155, China.
Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, China.
Genes (Basel). 2023 Sep 23;14(10):1847. doi: 10.3390/genes14101847.
As a member of the forkhead box L gene family, plays a significant role in gonadal development and the regulation of reproduction. During the evolution of deuterostome, whole genome duplication (WGD)-enriched lineage diversifications and regulation mechanisms occurs. However, only limited research exists on duplication in teleost or other vertebrate species. In this study, two paralogs, and , were identified in the transcriptome of spotted knifejaw (), which had varying expressions in the gonads. The was expressed higher in the ovary, while was expressed higher in the testis. Phylogenetic reconstruction, synteny analysis, and the molecular evolution test confirmed that and likely originated from the first two WGD. The expression patterns test using qRT-PCR and ISH as well as motif scan analysis revealed evidence of potentially functional divergence between the and paralogs in spotted knifejaw. Our results indicate that and may originate from the first two WGD, be active in transcription, and have undergone functional divergence. These results shed new light on the evolutionary trajectories of and and highlights the need for further detailed functional analysis of these two duplicated paralogs.
作为叉头框 L 基因家族的一员，在性腺发育和生殖调控中发挥重要作用。在后口动物的进化过程中，发生了全基因组复制（WGD）富集谱系多样化和调控机制。然而，仅有有限的研究涉及硬骨鱼类或其他脊椎动物物种中的 复制。在这项研究中，在斑点刀颌鲷（）的转录组中鉴定出了两个 基因的同源基因 和 ，它们在性腺中具有不同的表达模式。在卵巢中表达水平更高，而在睾丸中表达水平更高。系统发育重建、基因共线性分析和分子进化测试证实 和 可能起源于前两次全基因组复制。使用 qRT-PCR 和原位杂交以及基序扫描分析进行的表达模式测试为斑点刀颌鲷中的 和 同源基因的潜在功能分化提供了证据。我们的研究结果表明 和 可能起源于前两次全基因组复制，转录活跃，并经历了功能分化。这些结果为 和 的进化轨迹提供了新的视角，并强调需要对这两个复制的同源基因进行进一步的详细功能分析。
