College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao, 266071, China.
Dev Comp Immunol. 2022 Aug;133:104432. doi: 10.1016/j.dci.2022.104432. Epub 2022 May 6.
As one of the most valuable maricultured species, spotted knifejaw (Oplegnathus punctatus) has high popularity in eastern Asia. In recent years, diseases caused by Vibrio harveyi have brought huge economic losses in spotted knifejaw industry. To better understand the molecular mechanisms of immune response about V. harveyi resistance in spotted knifejaw, a comparative transcriptome analysis was performed on spleen tissues at five different time points post-infection (0, 12, 24, 48 and 72 hpi). A total of 4279 differentially expressed genes (DEGs) were identified. KEGG pathways analysis showed that multiple immune-related pathways were significant regulated, including Toll-like receptor signaling pathway, ECM-receptor interaction pathway, cytokine-cytokine receptor interaction pathway and hematopoietic cell lineage pathway. Weighted gene co-expression network analysis showed that several immune-related pathways of the highest correlation with 12 hpi (cor = 0.89, P = 7e-06) were significantly enriched. In addition, 12 hpi was a turning point for 7 gene clusters out of 9 that were divided according to gene expression patterns. Therefore, we speculated that 12 hpi might be a very critical time point for spotted knifejaw against V. harveyi infection. Additionally, qRT-PCR was carried out to validate the expressions of 12 DEGs. This study provided the first systematical transcriptome analysis of spotted knifejaw against V. harveyi. The results could help us better understand the dynamic immune responses of spotted knifejaw against bacterial infection, and provide useful information for antibacterial defense in spotted knifejaw industry as well.
作为最有价值的海水养殖品种之一，斑点鹦嘴鱼（Oplegnathus punctatus）在东亚地区广受欢迎。近年来，哈维弧菌（Vibrio harveyi）引起的疾病给斑点鹦嘴鱼产业带来了巨大的经济损失。为了更好地了解斑点鹦嘴鱼对哈维弧菌抗性的免疫反应的分子机制，我们在感染后 5 个不同时间点（0、12、24、48 和 72 hpi）对脾脏组织进行了比较转录组分析。共鉴定出 4279 个差异表达基因（DEGs）。KEGG 通路分析表明，多个免疫相关通路被显著调控，包括 Toll 样受体信号通路、ECM-受体相互作用通路、细胞因子-细胞因子受体相互作用通路和造血细胞谱系通路。加权基因共表达网络分析表明，与 12 hpi 相关性最高的几个免疫相关通路（cor=0.89，P=7e-06）显著富集。此外，12 hpi 是根据基因表达模式分为 9 个基因簇的 7 个基因簇的转折点。因此，我们推测 12 hpi 可能是斑点鹦嘴鱼抵抗哈维弧菌感染的一个非常关键的时间点。此外，还进行了 qRT-PCR 验证了 12 个 DEGs 的表达情况。本研究首次对斑点鹦嘴鱼抵抗哈维弧菌进行了系统的转录组分析。研究结果有助于我们更好地了解斑点鹦嘴鱼对细菌感染的动态免疫反应，并为斑点鹦嘴鱼产业的抗菌防御提供有用信息。
