Área de Bioquímica y Biología Molecular, Departamento de Biología Molecular, Universidad de León, 24007 León, Spain.
Genes (Basel). 2023 Sep 30;14(10):1897. doi: 10.3390/genes14101897.
strain U can be grown using, as sole carbon sources, the biogenic amines putrescine or cadaverine, as well as their catabolic intermediates, ɣ-aminobutyrate or δ-aminovalerate, respectively. Several paralogs for the genes that encode some of the activities involved in the catabolism of these compounds, such as a putrescine-pyruvate aminotransferase ( and genes) and a ɣ-aminobutyrate aminotransferase ( and genes) have been identified in this bacterium. When the expression pattern of these genes is analyzed by qPCR, it is drastically conditioned by supplying the carbon sources. Thus, is upregulated by putrescine, whereas seems to be exclusively induced by cadaverine. However, increases its expression in response to different polyamines or aminated catabolic derivatives from them (i.e., ɣ-aminobutyrate or δ-aminovalerate), although does not change its expression level concerning no-amine unrelated carbon sources (citrate). These results reveal differences between the mechanisms proposed for polyamine catabolism in and concerning strain U, as well as allow a deeper understanding of the enzymatic systems used by this last strain during polyamine metabolism.
U 株可以利用腐胺或尸胺作为唯一碳源进行生长，也可以利用它们的代谢中间产物 γ-氨基丁酸或 δ-氨基戊酸进行生长。在这种细菌中，已经鉴定出了一些基因的多个等位基因，这些基因编码参与这些化合物代谢的一些活性，例如腐胺-丙酮酸氨基转移酶（和 基因）和 γ-氨基丁酸氨基转移酶（和 基因）。通过 qPCR 分析这些基因的表达模式时，会受到碳源供应的极大影响。因此，腐胺会上调 基因的表达，而尸胺似乎只诱导 基因的表达。然而，该基因会响应不同的多胺或它们的氨基代谢衍生物（即 γ-氨基丁酸或 δ-氨基戊酸）而增加其表达，尽管对于与非胺无关的碳源（柠檬酸盐），其表达水平不会改变。这些结果揭示了 菌株 U 中多胺代谢的机制与 和 中提出的机制之间的差异，并允许更深入地了解该最后一种菌株在多胺代谢过程中使用的酶系统。
