Plant Research Team, Animal and Plant Research Department, Nakdonggang National Institute of Biological Resources, Sangju 37242, Republic of Korea.
Department of Agriculture, Forestry and Bioresources, Plant Genomics & Breeding Institute, Research Institute of Agriculture and Life Science, College of Agriculture & Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Genes (Basel). 2023 Oct 7;14(10):1914. doi: 10.3390/genes14101914.
Potamogetonaceae are aquatic plants divided into six genera. The largest genus in the family is , which is morphologically diverse with many hybrids and polyploids. Potamogetonaceae plastomes were conserved in genome size (155,863 bp-156,669 bp), gene contents (113 genes in total, comprising 79 protein-coding genes and 30 tRNA and 4 rRNA genes), and GC content (36.5%). However, we detected a duplication of the gene in the IR region of the and plastomes. A comparative analysis of Alismatales indicated that the plastomes of Potamogetonaceae, Cymodaceae, and Ruppiaceae have experienced a 6-kb inversion of the region and the complex has been lost in the plastome. Five divergent hotspots (, intron, , , and ) were identified among the plastomes, which will be useful for species identification. Phylogenetic analyses showed that the family Potamogetonaceae is a well-defined with 100% bootstrap support and divided into two different clades, and . Compared to the nucleotide substitution rates among Alismatales, we found neutral selection in all plastid genes of species. Our results reveal the complete plastome sequences of species, and will be helpful for taxonomic identification, the elucidation of phylogenetic relationships, and the plastome structural analysis of aquatic plants.
泽泻科植物分为六个属。该科最大的属是 ，形态多样，有许多杂种和多倍体。泽泻科植物质体在基因组大小（155863bp-156669bp）、基因含量（共 113 个基因，包括 79 个蛋白编码基因和 30 个 tRNA 和 4 个 rRNA 基因）和 GC 含量（36.5%）方面保持保守。然而，我们在 和 质体的 IR 区检测到了 基因的重复。对泽泻目植物的比较分析表明，泽泻科、慈姑科和眼子菜科的质体经历了 6kb 的 区倒位，而 复合体在 质体中丢失。在 质体中鉴定出五个分歧热点（、内含子、、、和 ），这将有助于物种鉴定。系统发育分析表明，泽泻科是一个明确的科，具有 100%的自展支持，并分为两个不同的分支，和 。与泽泻目植物的核苷酸取代率相比，我们发现 物种的所有质体基因都处于中性选择状态。我们的研究结果揭示了 种的完整质体序列，这将有助于分类鉴定、系统发育关系的阐明以及水生植物的质体结构分析。
