Alyahyawi Amjad R, Khan Mohd Yasir, Alouffi Sultan, Maarfi Farah, Akasha Rihab, Khan Saif, Rafi Zeeshan, Alharazi Talal, Shahab Uzma, Ahmad Saheem
Department of Diagnostic Radiology, College of Applied Medical Science, University of Hail, Ha'il 2440, Saudi Arabia.
Centre for Nuclear and Radiation Physics, Department of Physics, University of Surrey, Guildford GU2 7XH, UK.
Life (Basel). 2023 Sep 29;13(10):1986. doi: 10.3390/life13101986.
Methylglyoxal (MG) is a precursor for advanced glycation end-products (AGEs), which have a significant role in diabetes. The present study is designed to probe the immunological response of native and glycated low-density lipoprotein (LDL) in experimental animals. The second part of this study is to probe glycoxidative lesion detection in low-density lipoproteins (LDL) in diabetes subjects with varying disease duration. The neo-epitopes attributed to glycation-induced glycoxidative lesion of LDL in DM patients' plasma were, analyzed by binding of native and MG-modified LDL immunized animal sera antibodies using an immunochemical assay. The plasma purified human LDL glycation with MG, which instigated modification in LDL. Further, the NewZealand-White rabbits were infused with unmodified natural LDL (N-LDL) and MG-glycatedLDL to probe its immunogenicity. The glycoxidative lesion detection in LDL of DM with disease duration (D.D.) of 5-15 years and D.D. > 15 years was found to be significantly higher as compared to normal healthy subjects (NHS) LDL. The findings support the notion that prolonged duration of diabetes can cause structural alteration in LDL protein molecules, rendering them highly immunogenic in nature. The presence of LDL lesions specific to MG-associated glycoxidation would further help in assessing the progression of diabetes mellitus.
甲基乙二醛（MG）是晚期糖基化终产物（AGEs）的前体，而AGEs在糖尿病中起着重要作用。本研究旨在探究实验动物体内天然和糖基化低密度脂蛋白（LDL）的免疫反应。本研究的第二部分是探究不同病程的糖尿病患者低密度脂蛋白（LDL）中的糖氧化损伤检测。通过使用免疫化学分析，利用天然和MG修饰的LDL免疫动物血清抗体的结合，分析糖尿病患者血浆中归因于LDL糖基化诱导的糖氧化损伤的新表位。用MG对血浆纯化的人LDL进行糖基化，从而引发LDL的修饰。此外，给新西兰白兔注射未修饰的天然LDL（N-LDL）和MG糖基化的LDL，以探究其免疫原性。发现病程（D.D.）为5 - 15年和D.D.> 15年的糖尿病患者LDL中的糖氧化损伤检测结果与正常健康受试者（NHS）的LDL相比显著更高。这些发现支持了这样一种观点，即糖尿病病程延长会导致LDL蛋白质分子的结构改变，使其在本质上具有高度免疫原性。MG相关糖氧化特有的LDL损伤的存在将进一步有助于评估糖尿病的进展。
