Luo Hengming, Wang Qun, Dong Chao, Shi Zhufeng, He Chengxing, Guo Zhixiang, Shi Junyi, Li Chun, Gao Wei, Li Jinbin
Agricultural Environment and Resource Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Ministry of Agriculture and Rural Affairs International Joint Research Center for Agriculture, Kunming 650205, China.
Life (Basel). 2023 Oct 23;13(10):2101. doi: 10.3390/life13102101.
Bacterial leaf blight is a devastating disease of rice worldwide. The resistant genes are routinely transferred from landraces to cultivated varieties through backcross breeding along with marker-assisted selection. In the present study, we use the gene-specific markers to screen the rice landraces in Yunnan Province of China. We collected 404 representative samples of 24 different rice landraces from Yunnan Province of China. The initial PCR-based screening suggested that the leaf blight resistance was not evenly distributed in Yunnan Province. Our results indicate that there is a complete loss of resistance for landraces based on and genes. On the other hand, landraces harboring and showed a high level of resistance. Using gene-specific PCR-based data, we were able to identify the resistant, susceptible and heterozygous populations across Yunnan Province. The widely used gene alone showed a remarkable level of resistance throughout the province, indicating its potential to develop broad-spectrum resistance in rice germplasm. The key aspects of bacterial blight spread according to local sites in Yunnan Province and the resistance conferred by different landraces due to the presence of different resistance genes are discussed.
细菌性叶斑病是一种在全球范围内对水稻具有毁灭性的病害。抗性基因通常通过回交育种并结合标记辅助选择从地方品种转移到栽培品种中。在本研究中，我们使用基因特异性标记对中国云南省的水稻地方品种进行筛选。我们从中国云南省收集了24个不同水稻地方品种的404个代表性样本。基于PCR的初步筛选表明，叶斑病抗性在云南省分布不均。我们的结果表明，基于[具体基因1]和[具体基因2]基因的地方品种抗性完全丧失。另一方面，携带[具体基因3]和[具体基因4]的地方品种表现出高水平的抗性。利用基于基因特异性PCR的数据，我们能够识别云南省各地的抗性、易感和杂合群体。单独广泛使用的[具体基因5]基因在全省范围内表现出显著的抗性水平，表明其在水稻种质中开发广谱抗性的潜力。本文讨论了云南省当地细菌性叶斑病传播的关键方面以及不同地方品种因存在不同抗性基因而赋予的抗性。
