Yazid S N, Ahmad K, Razak M S F A, Rahman Z A, Ramachandran K, Mohamad S N A, Ghaffar M B Ab
Universiti Putra Malaysia - UPM, Faculty of Agriculture, Department of Plant Protection, Serdang, Selangor, Malaysia.
Universiti Putra Malaysia - UPM, Institute of Tropical Agriculture and Food Security - ITAFoS, Serdang, Selangor, Malaysia.
Braz J Biol. 2021 Nov 1;84:e248359. doi: 10.1590/1519-6984.248359. eCollection 2021.
Bacterial leaf blight (BLB) is one of the major rice diseases in Malaysia. This disease causes substantial yield loss as high as 70%. Development of rice varieties which inherited BLB resistant traits is a crucial approach to promote and sustain rice industry in Malaysia. Hence, this study aims were to enhance BLB disease resistant characters of high yielding commercial variety MR219 through backcross breeding approach with supporting tool of marker-assisted selection (MAS). Broad spectrum BLB resistance gene, Xa7 from donor parent IRBB7 were introgressed into the susceptible MR219 (recurrent parent) using two flanking markers ID7 and ID15. At BC3F4, we managed to generate 19 introgressed lines with homozygous Xa7 gene and showed resistant characteristics as donor parent when it was challenged with Xanthomonas oryzae pv. oryzae through artificial inoculation. Recurrent parent MR219 and control variety, MR263 were found to be severely infected by the disease. The improved lines exhibited similar morphological and yield performance characters as to the elite variety, MR219. Two lines, PB-2-107 and PB-2-34 were chosen to be potential lines because of their outstanding performances compared to parent, MR219. This study demonstrates a success story of MAS application in development of improved disease resistance lines of rice against BLB disease.
细菌性叶枯病（BLB）是马来西亚主要的水稻病害之一。这种病害会导致高达70%的大幅产量损失。培育具有抗BLB性状的水稻品种是促进和维持马来西亚水稻产业的关键途径。因此，本研究旨在通过回交育种方法并借助标记辅助选择（MAS）工具来增强高产商业品种MR219的抗BLB病特性。利用两个侧翼标记ID7和ID15，将来自供体亲本IRBB7的广谱抗BLB基因Xa7导入易感的MR219（轮回亲本）中。在BC3F4代，我们成功培育出19个带有纯合Xa7基因的渐渗系，当通过人工接种用稻黄单胞菌水稻致病变种进行挑战时，这些渐渗系表现出与供体亲本一样的抗性特征。发现轮回亲本MR219和对照品种MR263受到该病害的严重感染。改良系表现出与优良品种MR219相似的形态和产量性能特征。由于PB - 2 - 107和PB - 2 - 34这两个品系与亲本MR219相比表现出色，因此被选为潜在品系。本研究证明了MAS在培育改良的抗BLB病水稻品系中的成功应用。
