Bouallegue Amal, Thebti Siwar, Horchani Faouzi, Hosni Taoufik, Nouairi Issam, Mhadhbi Haythem, Trabelsi Najla, Amri Moez, Kharrat Mohamed, Abbes Zouhaier
Laboratoire des Grandes Cultures, Institut National de la Recherche Agronomique de Tunisie (INRAT), University of Carthage, Rue Hédi Karray, Menzah 1004, Tunisia.
Centre de Biotechnologie de Borj Cedria (CBBC), Laboratoire des Légumineuses et des Agrosys-Tèmes Durables, PB 901, Hammam Lif 2050, Tunisia.
Plants (Basel). 2023 Oct 15;12(20):3578. doi: 10.3390/plants12203578.
Broomrapes ( spp.) are root parasitic plants that threaten agricultural production in many parts of the world. In this study, the effect of two orobanche species, and , on faba bean plants was studied in Tunisia. The two orobanche species inhibited both biomass production and pod formation, decreased the chlorophyll (Chl) content and total lipid (TL), and enhanced electrolyte leakage (EL) and lipid peroxidation. Concomitantly, orobanche parasitism induced a lower degree of fatty acid (FA) unsaturation due to a shift in the FA composition. On the other hand, with regard to orobanche seeds, oleic and linoleic acids were the predominant FA in the two orobanche species. After orobanche seed germination and penetration of host tissues, all the orobanche development stages showed a decrease in the TL content and changes in the FA composition in comparison to orobanche seeds. The level of TL was equal to or lower in all parasite development stages (except for S4) than that in the roots and leaves of healthy faba bean plants. These results suggest that the negative effect of orobanche infestation on faba bean development can be attributed to the reduced chlorophyll content and alteration in membrane stability attested by the reduced TL level and FA unsaturation.
列当属植物是根部寄生植物，对世界许多地区的农业生产构成威胁。在本研究中，在突尼斯研究了两种列当属植物，即 和 ，对蚕豆植株的影响。这两种列当属植物均抑制生物量生产和豆荚形成，降低叶绿素（Chl）含量和总脂质（TL），并增加电解质渗漏（EL）和脂质过氧化。同时，由于脂肪酸（FA）组成的变化，列当属寄生导致脂肪酸不饱和度降低。另一方面，就列当属种子而言，油酸和亚油酸是这两种列当属植物中的主要脂肪酸。在列当属种子萌发并穿透宿主组织后，与列当属种子相比，列当属的所有发育阶段均显示总脂质含量降低且脂肪酸组成发生变化。在所有寄生发育阶段（S4除外），总脂质水平等于或低于健康蚕豆植株根和叶中的总脂质水平。这些结果表明，列当属侵染对蚕豆发育的负面影响可归因于叶绿素含量降低以及总脂质水平和脂肪酸不饱和度降低所证明的膜稳定性改变。
