Amkul Kitiya, Laosatit Kularb, Lin Yun, Yuan Xingxing, Chen Xin, Somta Prakit
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Plants (Basel). 2023 Oct 18;12(20):3602. doi: 10.3390/plants12203602.
Two bruchid species, and , are the most significant stored insect pests of tropical legume crops. Previously, we identified a major QTL, , controlling seed resistance to these bruchids in the cultivated zombi pea () accession 'TVNu 240'. In this study, we have narrowed down the region and identified a candidate gene conferring this resistance. Fine mapping using F and F populations derived from a cross between TVNu 240 and TVNu 1623 (susceptible) revealed the existence of two tightly linked QTLs, designated and , within the . The QTLs and explained 37.46% and 10.63% of bruchid resistance variation, respectively. was mapped to a 28.24 kb region containing four genes, from which the gene encoding a xylanase inhibitor was selected as a candidate gene responsible for the resistance associated with the . Sequencing and sequence alignment of from TVNu 240 and TVNu 1623 revealed a 1-base-pair insertion/deletion and five single-nucleotide polymorphisms (SNPs) in the 5' UTR and 11 SNPs in the exon. Alignment of the VvTAXI protein sequences showed five amino acid changes between the TVNu 240 and TVNu 1623 sequences. Altogether, these results demonstrated that the encoding xylanase inhibitor is the candidate gene conferring bruchid resistance in the zombi pea accession TVNu 240. The gene will be useful for the molecular breeding of bruchid resistance in the zombi pea.
两种豆象，即[豆象名称1]和[豆象名称2]，是热带豆类作物中最重要的仓储害虫。此前，我们在栽培的僵尸豆（[僵尸豆学名]）品种‘TVNu 240’中鉴定出一个控制种子对这些豆象抗性的主要数量性状位点（QTL），即[QTL名称]。在本研究中，我们缩小了[QTL名称]区域，并鉴定出一个赋予这种抗性的候选基因。利用TVNu 240和TVNu 1623（感虫品种）杂交产生的F[具体世代1]和F[具体世代2]群体进行精细定位，结果显示在[QTL名称]区域内存在两个紧密连锁的QTL，分别命名为[QTL名称1]和[QTL名称2]。[QTL名称1]和[QTL名称2]分别解释了豆象抗性变异的37.46%和10.63%。[QTL名称1]被定位到一个包含四个基因的28.24 kb区域，从中选择编码木聚糖酶抑制剂的[基因名称]作为与[QTL名称1]相关抗性的候选基因。对TVNu 240和TVNu 1623的[基因名称]进行测序和序列比对，结果显示在5'非翻译区有1个碱基对的插入/缺失以及5个单核苷酸多态性（SNP），在外显子中有11个SNP。VvTAXI蛋白序列比对显示TVNu 240和TVNu 1623序列之间有5个氨基酸变化。总之，这些结果表明编码木聚糖酶抑制剂的[基因名称]是赋予僵尸豆品种TVNu 240豆象抗性的候选基因。该基因将有助于僵尸豆抗豆象的分子育种。
