Novel Alleles of Two Tightly Linked Genes Encoding Polygalacturonase-Inhibiting Proteins (VrPGIP1 and VrPGIP2) Associated with the Locus That Confer Bruchid ( spp.) Resistance to Mungbean () Accession V2709.

Nearly all mungbean cultivars are completely susceptible to seed bruchids ( and ). Breeding bruchid-resistant mungbean is a major goal in mungbean breeding programs. Recently, we demonstrated in mungbean () accession V2802 that , which encodes a polygalacturonase inhibiting protein (PGIP), is the locus responsible for resistance to and . In this study, mapping in mungbean accession V2709 using a BCF population of 355 individuals revealed that a single major quantitative trait locus, which controlled resistance to both and , was located in a 237.35 Kb region of mungbean chromosome 5 that contained eight annotated genes, including () and (). and are located next to each other and are only 27.56 Kb apart. Sequencing and in "V2709" revealed new alleles for both VrPGIP1 and VrPGIP2, named and , respectively. has one single nucleotide polymorphism (SNP) at position 554 of wild type . This SNP is a guanine to cystine substitution and causes a proline to arginine change at residue 185 in the VrPGIP2 of "V2709". has 43 SNPs compared with wild type and "V2802", and 20 cause amino acid changes in VrPGIP1. One change is threonine to proline at residue 185 in VrPGIP1, which is the same as in VrPGIP2. Sequence alignments of VrPGIP2 and VrPGIP1 from "V2709" with common bean () PGIP2 revealed that residue 185 in VrPGIP2 and VrPGIP1 contributes to the secondary structures of proteins that affect interactions between PGIP and polygalacturonase, and that some amino acid changes in VrPGIP1 also affect interactions between PGIP and polygalacturonase. Thus, tightly linked and are the likely genes at the locus that confer bruchid resistance in mungbean "V2709".