Zhang Qi, Zhang Ying, Wang Yuhua, Zou Jishuang, Lin Shaoxiong, Chen Meihui, Miao Pengyao, Jia Xiaoli, Cheng Pengyuan, Pang Xiaomin, Ye Jianghua, Wang Haibin
College of Tea and Food, Wuyi University, Wuyishan 354300, China.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2023 Oct 20;12(20):3625. doi: 10.3390/plants12203625.
Pruning is an important agronomic measure in tea plantation management. In this study, we analyzed the effect of pruning on gene expression in tea leaves from a transcriptomics perspective and verified the results of a transcriptomic analysis in terms of changes in physiological indicators of tea leaves. The results showed that pruning enhanced the gene expression of nine metabolic pathways in tea leaves, including fatty acid synthesis and carbohydrate metabolism, nitrogen metabolism, protein processing in the endoplasmic reticulum, and plant hormone signal transduction, thereby promoting the growth of tea plants and increasing tea yield. However, pruning reduced the gene expression of nine metabolic pathways, including secondary metabolites biosynthesis, flavonoid biosynthesis, phenylpropanoid biosynthesis, and sesquiterpenoid and triterpenoid biosynthesis, and lowered the content of caffeine, flavonoids, and free amino acids in tea plant leaves. In conclusion, pruning could promote the growth of tea plants and increase the yield of tea, but it was not conducive to the accumulation of some quality indicators in tea leaves, especially caffeine, flavonoids, and free amino acids, which, in turn, reduced the quality of tea. This study provides an important theoretical reference for the management of agronomic measures in tea plantations.
修剪是茶园管理中的一项重要农艺措施。在本研究中，我们从转录组学角度分析了修剪对茶叶基因表达的影响，并从茶叶生理指标变化方面验证了转录组分析结果。结果表明，修剪增强了茶叶中9条代谢途径的基因表达，包括脂肪酸合成与碳水化合物代谢、氮代谢、内质网中的蛋白质加工以及植物激素信号转导，从而促进茶树生长并提高茶叶产量。然而，修剪降低了9条代谢途径的基因表达，包括次生代谢物生物合成、类黄酮生物合成、苯丙烷类生物合成以及倍半萜和三萜生物合成，并降低了茶树叶片中咖啡因、类黄酮和游离氨基酸的含量。总之，修剪可促进茶树生长并提高茶叶产量，但不利于茶叶中某些品质指标的积累，尤其是咖啡因、类黄酮和游离氨基酸，进而降低了茶叶品质。本研究为茶园农艺措施管理提供了重要的理论参考。
