Cárdenas-Aquino María Del Rosario, Camas-Reyes Alberto, Valencia-Lozano Eliana, López-Sánchez Lorena, Martínez-Antonio Agustino, Cabrera-Ponce José Luis
Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte Carr. Irapuato-León, Irapuato Gto 36824, Mexico.
Red de Estudios Moleculares Avanzados, Unidad de Microscopia Avanzada, Instituto de Ecología, A.C. INECOL 1975-2023, Carretera antigua a Coatepec 351, Col. El Haya, Xalapa 91073, Mexico.
Plants (Basel). 2023 Oct 21;12(20):3637. doi: 10.3390/plants12203637.
The known activities of cytokinins (CKs) are promoting shoot multiplication, root growth inhibition, and delaying senescence. 6-Benzylaminopurine (BAP) has been the most effective CK to induce shoot proliferation in cereal and grasses. Previously, we reported that in lemongrass () micropropagation, BAP 10 µM induces high shoot proliferation, while the natural CK 6-(γ,γ-Dimethylallylamino)purine (2-iP) 10 µM shows less pronounced effects and developed rooting. To understand the molecular mechanisms involved, we perform a protein-protein interaction (PPI) network based on the genes of involved in shoot proliferation/repression, cell cycle, stem cell maintenance, auxin response factors, and CK signaling to analyze the molecular mechanisms in BAP versus 2-iP plants. A different pattern of gene expression was observed between BAP- versus 2-iP-treated plants. In shoots derived from BAP, we found upregulated genes that have already been demonstrated to be involved in de novo shoot proliferation development in several plant species; CK receptors , stem cell maintenance , and ), cell cycle regulation ( complex), as well as the auxin response factor () and CK metabolism (). In contrast, in the 2-iP culture medium, there was an upregulation of genes involved in shoot repression (, ), , a type A-response regulator (), and auxin metabolism ().
细胞分裂素（CKs）已知的作用包括促进芽增殖、抑制根生长以及延缓衰老。6-苄基腺嘌呤（BAP）是诱导谷类和禾本科植物芽增殖最有效的细胞分裂素。此前，我们报道在柠檬草微繁殖中，10 μM的BAP能诱导高芽增殖，而10 μM的天然细胞分裂素6-(γ,γ-二甲基烯丙基氨基)嘌呤（2-iP）的效果则不那么明显，且能促进生根。为了解其中涉及的分子机制，我们基于参与芽增殖/抑制、细胞周期、干细胞维持、生长素反应因子和细胞分裂素信号传导的基因构建了蛋白质-蛋白质相互作用（PPI）网络，以分析BAP处理与2-iP处理植物中的分子机制。在BAP处理与2-iP处理的植物之间观察到了不同的基因表达模式。在源自BAP的芽中，我们发现一些已被证明参与多种植物从头芽增殖发育的基因上调；细胞分裂素受体、干细胞维持、以及、细胞周期调控（复合体），以及生长素反应因子和细胞分裂素代谢。相比之下，在2-iP培养基中，参与芽抑制的基因（、）、一种A型反应调节因子和生长素代谢上调。
